Duolink in situ pla probe anti goat plus

Manufactured by Merck Group

The Duolink In Situ PLA Probe Anti-Goat PLUS is a laboratory equipment product designed for protein-protein interaction analysis. It is a detection reagent that is used in conjunction with the Duolink In Situ PLA technology.

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Lab products found in correlation

Duolink in situ pla probe anti rabbit minus, by Merck Group (1 mentions) Probe anti mouse plus, by Merck Group (1 mentions) Duolink in situ pla probe anti mouse minus, by Merck Group (1 mentions) Duolink in situ pla, by Merck Group (1 mentions) Duolink in situ pla probe anti rabbit plus, by Merck Group (1 mentions) Oregon green 488, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Duolink in situ PLA Duolink PLA probes PLA Goat PLUS Duolink PLA PLA probes Anti-goat PLUS

2 protocols using duolink in situ pla probe anti goat plus

Antibody Validation for Protein Analysis

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All antibodies used in this study were validated by conventional immunoblotting (see Figs S1 and S5). See Table S1 for antibody source and dilution used for detection in the NanoPro1000 with and without RCA/PLA and by immunoblotting. Recombinant human VEGFA (293-VE-010) was purchased from R&D Systems.
Horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies were from ProteinSimple (#040-655, #040-656). The following secondary antibody-DNA conjugates were provided by Olink for Duolink In Situ PLA (the reagents are now available from Sigma Aldrich): Probe Anti-Mouse PLUS (Affinity Purified Donkey Anti-Mouse IgG, H+L; DUO92001), Duolink In Situ PLA Probe Anti-Rabbit PLUS (Affinity Purified Donkey Anti-Rabbit IgG, H+L; DUO92002), Duolink In Situ PLA Probe Anti-Goat PLUS (Affinity Purified Donkey Anti-Goat IgG, H+L; DUO92003), Duolink In Situ PLA Probe Anti-Mouse MINUS (Affinity Purified Donkey Anti-Mouse IgG, H+L; DUO92004), Duolink In Situ PLA Probe Anti-Rabbit MINUS (Affinity Purified Donkey anti-Rabbit IgG, H+L; DUO92005), Duolink In Situ PLA Probe Anti-Goat MINUS (Affinity Purified Donkey Anti-Goat IgG, H+L; DUO92006).

Padhan N., Yan J., Boge A., Scrivener E., Birgisson H., Zieba A., Gullberg M., Kamali-Moghaddam M., Claesson-Welsh L, & Landegren U. (2017). Highly sensitive and specific protein detection via combined capillary isoelectric focusing and proximity ligation. Scientific Reports, 7, 1490.

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Proximity Ligation Assay for Evaluating Fatty Acid Binding Interactions

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On day 0, cells were seeded at 60,000 cells per well containing a 12-mm round coverslip in 24-well plates. On day 1, cells were switched to medium supplemented with 5% delipidated FCS and 1 µM A939572 (SCD1 inhibitor) for 24 h. On day 2, cells treated with or without 50 µM ω-alkynyl AA and 150 µM AA for 4 h were subjected to click reaction to covalently attach Oregon Green 488 (Thermo Fisher Scientific) to the clickable analog of AA followed by proximity ligation assay as previously described (32 (link)), using 10 µg/mL rabbit anti-FAF1 and goat anti-fluorescein/Oregon Green (Thermo Fisher Scientific) as the primary antibody and Duolink in situ PLA probe anti-rabbit MINUS and Duolink in situ PLA probe anti-goat PLUS (Sigma-Aldrich) as the oligonucleotide-conjugated secondary antibody. Fluorescent images were acquired with excitation and emission wave lengths set at 531 and 592 nm, respectively. ImageJ software was used for quantification of the interaction signal.
Materials and other methods can be found in SI Appendix, SI Materials and Methods.

Cui S., Simmons G J.r., Vale G., Deng Y., Kim J., Kim H., Zhang R., McDonald J.G, & Ye J. (2022). FAF1 blocks ferroptosis by inhibiting peroxidation of polyunsaturated fatty acids. Proceedings of the National Academy of Sciences of the United States of America, 119(17), e2107189119.

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