Lubrol px

GANT61 (G9048), cyclopamine, protease inhibitor cocktail, and Lubrol-PX were purchased from Sigma-Aldrich (St. Louis, MO, USA). DMSO was purchased from Amresco and was used as the solvent for some reagents and the vehicle control. Puromycin was purchased from Salarbio. Doxycycline was purchased from Sangon Biotech (Shanghai, China). The primary antibody against Gli1 (2643S) was purchased from Cell Signaling (CST); the primary antibody against Kif3a (ab11259) were purchased from Abcam. The primary antibody against MMP-7 (C0273) was purchased from ANBO; antibody against Gli2 (sc28674) was purchased from Santa Cruz; antibody against GAPDH (MAB374) was purchased from Millipore. Enhanced chemiluminescence (ECL) Western blot detection reagents were purchased from Thermo Fisher Scientific Inc. The BLOCK-iT Pol II miR RNAi Expression Vector Kit (K4936-00) and Lipofectamine 2000 (11668-019) were purchased from Invitrogen.

Lubrol-PX and other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). Lipofectamine 2000 was obtained from Invitrogen Life Technologies (Carlsbad, CA, USA). The sources for monoclonal antibodies are as follows: Sigma-Aldrich (anti-Flag M2, F3165; anti-c-Myc, M4439), Abcam (anti-Pathed/PTCH1, ab55629; anti-SuFu, ab52913), Santa Cruz Biotechnology (normal rabbit IgG, sc-2027; anti-Gli2, sc-271786), BD Pharmingen (anti-Nek2 MAB, 610594), Covance (anti-ubiquitin P4G7, MMS-258R), and Millipore (anti-GAPDH, MAB374). Polyclonal antibodies were purchased from Abcam (anti-SuFu, ab28083; anti-Smo, ab38686; anti-Gli1, ab92611). The working concentrations for small molecular inhibitors and chemicals are as follows: GANT61 (20 mM, G9048; Sigma-Aldrich), MG-132 (25 mM, S2619; Selleck), purmorphamine (20 mM, S3042; Selleck), CHX (100 mg/ml, C7689; Solarbio). DMSO (0231; Amresco) was used as the solvent for the inhibitors and the vehicle control.