Vaginal temperature was recorded every 30 min over a 48-h period at 23 and 9 d BEC and 14 and 60 d PP using internal temperature loggers (iButton DS1921H-F5#, iButtonLink Technology) secured to a blank controlled internal drug release device (EAZI-Breed CIDR Cattle Insert, Zoetis Inc.) and inserted into the vagina.
Eazi breed cidr cattle insert
Manufactured by Zoetis
Sourced in United States
The EAZI-Breed CIDR Cattle Insert is a specialized laboratory equipment designed for use in cattle breeding and management. It serves as a controlled internal drug release (CIDR) device, providing a consistent and controlled release of progesterone for synchronization of the estrous cycle in cattle.
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Lab products found in correlation
7 protocols using eazi breed cidr cattle insert
1
Vaginal Temperature Monitoring Protocol
2
Tracking Bovine Body Temperature and Resting Behavior
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Calibrated temperature loggers (iButton DS1921H-F5#, iButtonLink Technology, Baulkham Hills, Australia) were used to measure body temperature (Burfeind et al., 2011 (link)(Burfeind et al., , 2014)) (link). Each iButton was attached to a blank controlled internal drug release device (EAZI-Breed CIDR Cattle Insert, Zoetis Inc., Parsippany-Troy Hills, NJ). On d 23 and 9 BEC (average of 21 and 7 d before calving ± 3.7 d), starting at 0430 h, iButton controlled internal drug release devices were inserted into the vagina for 48 h and set to record temperature every 30 min (Burdick et al., 2012; (link)Johnson and Shade, 2017) (link).
Following twice-daily exercise, cows were herded through the milking parlor at 0500 h (primarily darkphase recording for control) and 1600 h (primarily light-phase recording for control) to collect the resting data from individual pedometers (AfiAct II Leg Tag, Afimilk USA Inc., Fitchburg, WI). Pedometers were attached when cows were enrolled into the study at 35 d BEC and were used to record resting bouts (number of bouts), rest duration (average minutes of resting per bout), and total resting time (minutes) up to 15 DIM. To calculate the total daily rest duration, dark-and light-phase recordings were averaged. Daily data for total resting time and resting bouts were calculated as the summation of dark-and light-phase recordings.
Following twice-daily exercise, cows were herded through the milking parlor at 0500 h (primarily darkphase recording for control) and 1600 h (primarily light-phase recording for control) to collect the resting data from individual pedometers (AfiAct II Leg Tag, Afimilk USA Inc., Fitchburg, WI). Pedometers were attached when cows were enrolled into the study at 35 d BEC and were used to record resting bouts (number of bouts), rest duration (average minutes of resting per bout), and total resting time (minutes) up to 15 DIM. To calculate the total daily rest duration, dark-and light-phase recordings were averaged. Daily data for total resting time and resting bouts were calculated as the summation of dark-and light-phase recordings.
3
Synchronizing Estrus in Dairy Cows
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Estrus was synchronized in all cows using a Select-Synch+ CIDR protocol. Briefly, a 1.3 g progesterone intravaginal insert (CIDR, Eazi-Breed™ CIDR® Cattle Insert; Zoetis Animal Health, New York, NY, USA) was placed in the vagina and a 100 µg of gonadorelin diacetate tetrahydrate (GnRH; 2 mL; im; Cystorelin®, Merial Inc., Duluth, GA, USA) was administered on Day-10. The CIDRs were removed, estrus alert patches (Western Point Inc., Apple Valley, MN, USA) were fitted, and 25 mg of dinoprost (PGF2α; 5 mL; im; Lutalyse® sterile solution; Zoetis Animal Health) was administered to all cows on Day −3. Cows were observed thrice daily either for standing estrus or for the status of the estrus detector aid up to 96 h. A cow was designated as in estrus if she was visually observed to stand for mounting by her herd-mates or if she had an activated, lost (with mount marks), or partially activated heat detector aid (Day 0).
4
Synchronizing Bovine Estrous Cycle for TAI
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To determine cyclicity, blood samples were collected from all cows on d -7 and d 0 (Fig. 1 ). On d 0, GnRH (100 μg, i.m.; Factrel; Zoetis, Kalamazoo, MI) was administered to all animals and a CIDR (1.38 g [P4]; EAZI-breed CIDR cattle insert; Zoetis) was inserted (Fig. 1 ). On d 5, CIDR inserts were removed and cows were stratified by BCS, age, and DPP and randomly assigned to receive either one 25 mg dose of HighCon (i.m., n = 100; Lutalyse HighCon; Zoetis) or two 25 mg doses of conventional PGF2⍺ (i.m., n = 100; Lutalyse; Zoetis) 7 to 11 h apart. On d 8, all cows received a second GnRH and simultaneous TAI with semen from six sires by two trained technicians. A final blood sample was collected on d 8 for P4 analysis (Fig. 1 ). All cows were exposed to bulls 5 to 14 d after AI.
5
Fixed-Time AI in Angus-Brahman Crossbred Heifers
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Data were collected on a population of Angus–Brahman crossbred heifers owned by the Seminole Tribe of Florida Inc., located in Northeast Glades, Florida, USA (27°04’ N 81°04’ W) (n = 2,272; average Brahman percentage = 23%; range 1% to 59%). Body weight at 4 months before breeding averaged 357 kg (range 229 to 528 kg). In November of each year of the study (2016–2018), heifers, approximately 2 years of age, were subjected to fixed-time AI using a 5-day Co-Synch + CIDR protocol. On d 0 of the protocol, heifers were administered 100 µg gonadotropin releasing hormone (GnRH) intramuscularly and a CIDR device containing 1.38 g progesterone (Eazi-Breed CIDR Cattle Insert; Zoetis Inc., Madison, NJ, USA) was inserted intravaginally. On d 5, the CIDR was removed and 25 mg prostaglandin F2α (PGF2α) was administered intramuscularly. Approximately 8 h after the first dose of PGF2α, another equal dose was administered. On d 8, immediately prior to AI, heifers received 100 µg GnRH intramuscularly (66 ± 2 h after CIDR removal). Each heifer was inseminated one time and then heifers were placed with bulls ~ 14 d later for natural mating for 90 d. Ultrasonic examination of the reproductive tract was conducted twice to determine pregnancy status at ~ 30 d after AI and again at the end of the breeding season.
6
Thermal Stress Effects on Embryo Yield
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Cows in the CON group (n = 100) were experienced THI of < 73 in early spring for 3 weeks prior to initiation of synchronization protocol, whereas cows in the HS group (n = 100) exposed to a THI of ≥ 73 in summer months for 3 weeks prior to initiation of synchronization protocol (-Day 21)10 (link). A CO-Synch + CIDR protocol (Fig. 4 ) was used to synchronize all cows, similar to the experiment 1a. The AI sires (n = 4) were randomly allocated to donor cows. The SCR score of the AI sires were ≥ + 4.![]()
The schematic presentation of CIDR + CO-Synch protocol. All cows were fitted with a 1.3 g progesterone intravaginal insert (CIDR, Eazi-Breed CIDR Cattle Insert; Zoetis Animal Health, New York, NY, USA) and received 100 µg of gonadorelin hydrochloride (GnRH; 2 mL; im, Factrel; Zoetis Animal Health) on Day-10. On Day-3, CIDRs were removed, and 25 mg of dinoprost (PGF2α; 5 mL; im; Lutalyse sterile solution; Zoetis Animal Health) was administered to all cows. Cows in estrus were inseminated once 66 h after CIDR removal and administered with 100 µg of GnRH (im, Zoetis Animal Health) concomitantly (Day 0). Embryos were recovered by non-surgical uterine flush technique on Day 16. Rectal temperatures were recorded on Day-10, -3 and 7, and blood was collected on Day 16.
7
Synchronized Estrus Cycle in Holstein-Friesian Heifers
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Eight Holstein-Friesian heifers (15–17 months old) were oestrus-synchronised using Eazi-Breed™ CIDR® Cattle Insert (1.38 g progesterone over eight days; Zoetis, USA), Receptal® (0.02 mg buserelin on the day of CIDR insertion; MSD Animal Health, UK) and Estrumate® (0.5 mg cloprostenol seven days after CIDR insertion; MSD Animal Health). Blood was collected on Days 0, 8 and 16 from all animals (Day 0 being the first day of observed oestrus). The stage of the oestrous cycle was confirmed by plasma progesterone profiles determined using a Coat-a-Count radio-immuno-assay (Siemens Healthcare, Germany). All animal procedures were carried out under the UK Home Office Animals (Scientific Procedures) Act 1986, license 60/4604, and with approval by the Ethical Review Committee, University of Edinburgh and all efforts were made to minimize animal suffering.
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