Vero E6 cells (ATCC® number 1568) were purchased from the American Type Culture Collection (ATCC®). Dulbecco’s Eagle medium (DMEM) and fetal bovine serum (FBS), streptomycin and penicillin, HEPES, agarose, paraformaldehyde, crystal violet, PBS, and DMSO were purchased from Sigma-Aldrich (Gillingham, UK). Dipeptide library was purchased from Pepmic Co Ltd. (Suzhou, China).
Dulbecco s eagle medium
Manufactured by Merck Group
Sourced in United Kingdom
Dulbecco's Eagle medium is a cell culture medium used for the in vitro maintenance and growth of various cell types. It provides the necessary nutrients and components to support cell proliferation and survival.
Automatically generated - may contain errors
Lab products found in correlation
Phosphate buffered saline (pbs), by Merck Group (2 mentions)
Crystal violet, by Merck Group (1 mentions)
Vero e6 cell, by American Type Culture Collection (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
Agarose, by Merck Group (1 mentions)
Hepes, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by HiMedia (1 mentions)
Sodium hydroxide (naoh), by HiMedia (1 mentions)
Human tnf α, by Thermo Fisher Scientific (1 mentions)
Trichloroacetic acid, by Merck Group (1 mentions)
Griess reagent, by Promega (1 mentions)
Ethanol, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Sucrose, by Merck Group (1 mentions)
Calcium chloride, by Merck Group (1 mentions)
Picric acid, by Merck Group (1 mentions)
3 protocols using dulbecco s eagle medium
1
Vero E6 Cells Dipeptide Assay
2
Comprehensive Sourcing of Research Reagents
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All the chemicals, as well as reagents, are of high purity. Dulbecco’s eagle medium (DMEM) and phosphate buffer saline (PBS) were purchased from Sigma, UK. Elisa kits, IL-6, Human TNF-α, were purchased from Invitrogen (USA). From Gibco USA, fetal bovine serum was obtained and lipopolysaccharide (LPS) E. Coli from Calbiochem (USA). Griess reagent was purchased from Promega (USA) and MTT from Calbiochem (San Diego CA). Besides, other chemicals that were used were of research-grade. From the central drug house (CDH) Jammu, ferric chloride, glacial acetic acid, chloroform was purchased. Ascorbic acid, ethanol, methanol, picric acid, calcium chloride, sodium chloride, 2,2 diphenyl picryl hyradyzl (DPPH), sucrose, trichloroacetic acid were procured from Merck. From Qualigens, potassium dihydrogen phosphate, hydrogen peroxide and hydrochloric acid were purchased. Ferric nitrate, ethylene diamine tetraacetate, potassium chloride, sodium hydroxide, dimethyl sulfoxide, sodium dihydrogen monophosphate was purchased from HiMedia. From sisco research laboratories (SRL) butylated hydroxytoluene was bought and from Rankem ethyl acetate and hexane was purchased.
3
Benzyl Isothiocyanate Cytotoxicity Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Benzyl Isothiocyanate, β-CD, hp-β-CD, hexane, Dulbecco's eagle medium (DMEM), Phosphate Buffered Saline, were procured from Sigma-Aldrich, purity > 99%), while Ethanol (absolute) was obtained from Changshu Yangyuan Chemical (China). Millipore water with conductance less than 3 µS cm-1 was used for all the preparations. MDA-MB-231cells were obtained from NCCS, Pune, India.
Fetal bovine serum (FCS), Antibiotic/antimycotic cocktail, Trypsin-Ethylenediaminetetraacetic acid (EDTA) solution, L-Glutamine, MTT, Propidium Iodide (PI) were purchased from Himedia, India. Annexin-PI apoptosis kit was procured from Thermo Fisher Scientific, USA.
Fetal bovine serum (FCS), Antibiotic/antimycotic cocktail, Trypsin-Ethylenediaminetetraacetic acid (EDTA) solution, L-Glutamine, MTT, Propidium Iodide (PI) were purchased from Himedia, India. Annexin-PI apoptosis kit was procured from Thermo Fisher Scientific, USA.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!