Ion images of selected m/z values were generated using the MIRION software package [66 (link)] with a bin width of ∆m/z = ±5 ppm [66 (link)]. The ion images were scaled to the highest intensity for each substance separately. RGB images of three different m/z values were overlaid and displayed simultaneously. Compounds were assigned based on high mass accuracy (root mean square error <3 ppm). For the analysis of tandem mass spectra, Mass Frontier™ version 7.0 (Thermo Fisher Scientific GmbH, Bremen, Germany) was used [67 ].
Mass frontier version 7
Manufactured by Thermo Fisher Scientific
Sourced in Germany
Mass Frontier™ version 7.0 is a software tool designed for the analysis and interpretation of mass spectrometry data. It provides a suite of analytical capabilities to support the processing and visualization of mass spectrometry results.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mass frontier version 7
1
Ion Image Generation and Analysis
2
Quantification of PQA-11 in Mouse Brain
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were treated with PQA-11 (0.5 mg/kg) by intraperitoneal single injection, brain tissues were prepared at 2, 4, and 6 h after the PQA-11 injection. PQA-11 in the brain tissues was analyzed by C18 reverse-phase liquid chromatography coupled to high resolution Mass spectrometry (MS) as described previously [22] . Briefly, PQA-11 was extracted by the method of Bligh and Dyer [33] . For detection of PQA-11, the extracts were analyzed on high performance liquid chromatography (LC)-MS/MS system (LC, UltiMate 3000; MS, TSQ Vantage and Orbitrap, Thermo Fisher Scientific) controlled by XCALIBUR. The m/z list of PQA-11 was obtained from Mass Frontier (version 7.0, Thermo Fisher Scientific) software for the prediction of PQA-11 fragments. The peak area of PQA-11 as a parent compound was calculated from mass spectra obtained by positive acquisition polarity in a mass spectrometer.
To assess relative concentrations of PQA-11, the peak area of PQA-11 was normalized to that of quinine added as an internal standard.
To assess relative concentrations of PQA-11, the peak area of PQA-11 was normalized to that of quinine added as an internal standard.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!