6-isopropyl dithio-2′-dexoxyguanosine (YLS004) and 6-isopropyl dithio-2′-guanosine (YLS010) were obtained from Chemipanda Bio-Tech Co., Ltd. (Hangzhou, China). 6-Thioguanine(6-TG) was purchased from Aladdin (Shanghai, China). Nelarabine was purchased from Market-Guide Pharmaceutical & Chemical Co., Ltd. (Jiangxi, China). Anti-BAX (Abcam, Cambridge, UK, Cat# ab182733, dilution ratio: 1:2000). Anti-BCL-2(Abcam, Cambridge, UK, Cat# ab32124, dilution ratio: 1:2000). Anti-Caspase 3 (Proteintech, Chicago, IL, USA, Cat# 19677-1-AP, dilution ratio: 1:1000). Anti-Caspase9 (Proteintech, Cat# 10380-1-AP, dilution ratio: 1:1000). Anti-GPX4 (Proteintech, Cat# 67763-1-lg, dilution ratio: 1:2000). Anti-ACSL4 (Proteintech, Cat# 66617-1-lg, dilution ratio: 1:5000). Anti-GAPDH (Proteintech, Cat# 60004-1-lg, dilution ratio: 1:5000).
Anti acsl4
Manufactured by Proteintech
Sourced in China
Anti-ACSL4 is an antibody product used for the detection and analysis of the ACSL4 protein. ACSL4 is an enzyme involved in fatty acid metabolism. The antibody can be used in various analytical techniques, such as western blotting, immunohistochemistry, and immunocytochemistry, to study the expression and localization of the ACSL4 protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti gpx4, by Proteintech (3 mentions)
Anti gapdh, by Proteintech (2 mentions)
Anti bax, by Abcam (1 mentions)
Anti caspase 3, by Proteintech (1 mentions)
Anti caspase 9, by Proteintech (1 mentions)
Anti bcl 2, by Abcam (1 mentions)
Ipvh00010, by Merck Group (1 mentions)
Bl520b, by Biosharp (1 mentions)
Ab244205, by Abcam (1 mentions)
Anti cox2, by Proteintech (1 mentions)
Anti dhodh, by Proteintech (1 mentions)
As1008, by Aspen (1 mentions)
Pc0020, by Solarbio (1 mentions)
Anti actin, by Proteintech (1 mentions)
Anti fsp1, by Cell Signaling Technology (1 mentions)
Anti slc7a11, by Proteintech (1 mentions)
Anti calnexin, by Proteintech (1 mentions)
Anti tsg101, by Proteintech (1 mentions)
Anti cd9, by Proteintech (1 mentions)
Anti cd63, by Proteintech (1 mentions)
4 protocols using anti acsl4
1
Investigating Ferroptosis Regulators in Cells
2
Proteomic Analysis of Ferroptosis Regulators
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In a solution containing a protease inhibitor (AS1008; Aspen), PC12 cells were dissolved on ice for 30 min. BCA protein assay kit was used to measure the protein concentration (PC0020; Solarbio). The protein samples were heated at 100°C for 10 min to completely denature the proteins. The protein samples were then separated by SDS‐PAGE electrophoresis and transferred to PVDF membranes (IPVH00010; Millipore). After being blocked for an hour with 5 percent skim milk, the membrane was incubated with the primary antibody at 4°C overnight. The antibodies used in this study consisted of anti‐DHODH (1:1000, 14877‐1‐AP; Proteintech), anti‐ACSL4 (1:1000, 22401‐1‐AP; Proteintech), anti‐COX2 (1:1000, 66351‐1‐Ig; Proteintech), anti‐GPX4 (1:1000, 67763‐1‐Ig; Proteintech), anti‐ALOX15 (1:1000, ab244205; Abcam), anti‐P53 (1:1000, AB_297667; Abcam). After being washed three times with TBST, the membrane was incubated for 10 min at room temperature with a secondary antibody that had been enzyme‐labeled. Imaging was carried out using an ECL chemiluminescence substrate (BL520B; Biosharp). Band intensities were quantified using Image J software.
3
Western Blot Analysis of Fibrosis Markers
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Protein expression of FAP, α-SMA, and FSP1 was assessed by protein blot analysis and samples were normalized to GAPDH or ACTIN. Samples were blocked with 5% skim milk powder for 2 h at room temperature and incubated at 4°C with anti-FAP (1:1000, Proteintech), anti-α-SMA (1:2000, Cell Signaling Technology), anti-FSP1 (1:1000, Cell Signaling Technology), anti-Calnexin (1:1000, Proteintech), anti-TSG101 (1:1000, Proteintech), anti-CD63 (1:1000, Proteintech), anti-CD9 (1:1000, Proteintech), anti-GAPDH (1:5000, Proteintech), anti-NFR2 (1:1000, Proteintech), anti-ACSL4 (1:1000, Proteintech), anti-SLC7A11 (1:1000, Proteintech), anti-CHAC1 (1:500, Proteintech), anti-GPX4 (1:1000, Proteintech) and anti-ACTIN (1:5000, Proteintech) antibodies overnight. Next, membranes were imaged with horseradish peroxidase-conjugated secondary antibodies (anti-rabbit or anti-mouse, 1:5000, Proteintech), and protein blots were imaged using a gel imaging system (Tanon) and quanti ed using the ImageJ software.
4
Immunohistochemical Staining of Fatty Acid Metabolism Proteins
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IHC staining was conducted according to the manufacturer's instructions using the Histostain™ Plus Kit (Cat. No. SP-9001; ZSGB-BIO, Beijing, China). The primary antibodies used in this study were anti-PPARα (1:200, Cat. No. ab233078; Abcam, Cambridge, UK), anti-ACADM (1:200, Cat. No. 55210-1-AP; Proteintech, China), anti-ACAA1(1:50, Cat. No. 12319-2-AP; Proteintech, China), anti-ACSL1(1:200, Cat. No. 13989-1-AP; Proteintech, China), anti-ACSL4 (1:50, Cat. No. 22401-1-AP; Proteintech, China) and anti-ACSL5(1:100, Cat. No. A1270, Abclonal, China). Positive signals for PPARα-, ACADM-, ACAA1-, ACSL1-, ACSL4-, and ACSL5-were observed as granular yellow or brown masses in hepatocytes.
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