Spinosin (6-(2-O-β-D-glucopyranosyl-β-D-glucopyranosyl)-5-hydroxy-2-(4-hydroxyphenyl)-7-methoxy-4H-1-benzopyran-4-one) was purchased from Meilunbio (Dalian, China), with HPLC purity 98%. Dulbecco’s modified Eagle’s medium (DMEM, high glucose) was purchased from HyClone (Logan, UT, USA), fetal bovine serum was purchased from Clark (Richmond, VA, USA), Opti-MEM and G418 disulfate salt were from Gibco (Grand Island, NY, USA). Human Aβ1-42 ELISA kit was purchased from Shanghai MLBIO Biotechnology (Shanghai, China). 2, 7-dichlorofluorescein diacetate (DCFH-DA) and BCA protein assay kit were purchased from Meilunbio. Thioflavin T (ThT) was from Absin Bioscience Inc (Shanghai, China). Rabbit anti-APP antibody and anti-BACE1 antibody were obtained from Cell Signaling Technology Inc (Danvers, MA, USA). Anti-Nrf2, β-actin, HO-1 and ADAM10 antibodies were purchased from Proteintech Inc (Chicago, IL, USA). Other reagents were cultured grade purity.
Anti bace1 antibody
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-BACE1 antibody is a laboratory reagent used to detect and quantify the BACE1 protein. BACE1 is an enzyme involved in the production of amyloid-beta peptides, which are associated with Alzheimer's disease. This antibody can be used in various analytical techniques, such as western blotting and immunohistochemistry, to study the expression and localization of BACE1 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Clark Bioscience (1 mentions)
Opti mem, by Thermo Fisher Scientific (1 mentions)
β actin, by Proteintech (1 mentions)
Bca protein assay kit, by Meilun (1 mentions)
Anti nrf2, by Proteintech (1 mentions)
Adam10, by Proteintech (1 mentions)
Tissue culture reagents, by Thermo Fisher Scientific (1 mentions)
6e10 against aβ1 16, by Fortrea (1 mentions)
Anti β actin antibody, by Abcam (1 mentions)
2 protocols using anti bace1 antibody
1
Spinosin Modulates Amyloid-beta Pathology
2
Amyloid-beta Aggregation and Clearance Assay
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Antibodies used included 6E10 against Aβ1-16 (Covance), 4G8 against Aβ17-24 (Covance), anti-BACE1 antibody (Cell Signalling), anti-neprilysin antibody (Santa Cruz), anti-insulin degrading enzyme (IDE) antibody (Abcam), anti-ANXA1 antibody (Zymed), anti-β-actin antibody (Abcam), anti-IgG antibody (Abcam), anti-FPRL1/FPR2 antibody (Acris Antibodies GmbH) and anti-IgG FITC conjugated antibody (AbD Serotec). Full-length human recombinant (hr) ANXA1 was obtained as previously described [16 (link)], and protein was purified by GTP Technology (Labege Cedex). Synthetic Aβ1–42, 5-FAM-labelled Aβ1–42 and Aβ1–42 scrambled peptides were obtained from Anaspec. Synthetic Aβ1–42 was prepared by suspension of the lyophilised Aβ1–42 in DMSO to 500 μM and then diluted to different concentrations ranging from 0.1 to 3 μM with cold DMEM. FPR2 inhibitors WRW4 and Boc-1 were obtained from Tocris Bioscience. Tissue culture reagents were obtained from Invitrogen. All other chemicals and reagents were purchased from Sigma-Aldrich, Qiagen and Invitrogen.
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