Primary antibody for phospho akt

Manufactured by Cell Signaling Technology

Sourced in United States

The primary antibody for phospho-AKT is a reagent used in laboratory research. It specifically binds to the phosphorylated form of the AKT protein, which is a key signaling molecule involved in various cellular processes. This antibody can be used to detect and quantify the levels of activated AKT in biological samples, such as cell lysates or tissue extracts, using techniques like Western blotting or immunohistochemistry.

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Lab products found in correlation

Limulus amebocyte lysate assay kit, by Lonza (1 mentions) Polymyxin b agarose beads, by Merck Group (1 mentions) Total akt, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Phospho-Akt (892 citations) Akt antibody (71 citations) Phospho-Akt antibody (21 citations)

2 protocols using primary antibody for phospho akt

Purification and Validation of S. japonicum SEA

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SEA of S. japonicum were obtained from Jiangsu Institute of Parasitic Diseases (China). SEA was sterile-filtered and endotoxin was removed with Polymyxin B agarose beads (Sigma, USA). Limulus amebocyte lysate assay kit (Lonza, Switzerland) was used to confirm the removal of endotoxins from the SEA as previously described [19 (link)]. Primary antibodies for FoxO3a, SKP2, P27 and AKT were purchased from Santa Cruz Biotechnology (USA, antibody dilution for Western blot of all antibodies from this company is 1:200). Primary antibody for phospho-AKT was purchased from Cell Signaling Technology (USA, antibody dilution for Western blot is 1:1000). All of the secondary antibodies were obtained from Santa Cruz Biotechnology (USA, antibody dilution is 1:2000). The staining kit for SA-β-Gal was purchased from GenMed Scientifics Inc (USA).

Duan Y., Pan J., Chen J., Zhu D., Wang J., Sun X., Chen L, & Wu L. (2016). Soluble Egg Antigens of Schistosoma japonicum Induce Senescence of Activated Hepatic Stellate Cells by Activation of the FoxO3a/SKP2/P27 Pathway. PLoS Neglected Tropical Diseases, 10(12), e0005268.

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Insulin Signaling Pathway Evaluation

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Ten minutes prior to sacrifice, a subset of animals (n = 4–5 per group) underwent intra-peritoneal injection of insulin (10 U/kg) or saline vehicle. Liver and gastrocnemius muscle were harvested, and tissues were homogenized in buffer containing 0.25 m sucrose, 10 mm Tris–HCl, and 0.1 mm EGTA and protease inhibitors. For each sample, 30 μg of protein was transferred to nitrocellulose membranes, which were incubated overnight with primary antibody for phospho-AKT (1 : 000; Cell Signaling Technology, Danvers, MA, USA) and total AKT (1 : 1000; Cell Signaling Technology). Membranes were washed, incubated with horseradish protein-conjugated secondary antibody, and developed with ECL reagent (Pierce, Rockford, IL, USA).

Rubinow K.B., Wang S., den Hartigh L.J., Subramanian S., Morton G.J., Buaas F.W., Lamont D., Gray N., Braun R.E, & Page S.T. (2015). Hematopoietic androgen receptor deficiency promotes visceral fat deposition in male mice without impairing glucose homeostasis. Andrology, 3(4), 787-796.

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