Briefly, cells in culture plates (Costar; Corning, NY, USA) were first fixed with 4% paraformaldehyde for 20 min at room temperature and then permeabilized with 0.2% Triton X-100 in PBS for 15 min. Fixed cells were washed with PBS and blocked with 5% goat serum in PBS for 1 h at 37°C to prevent nonspecific binding. Cells were incubated with corresponding primary antibodies at 37°C for 1 h and stained with Alexa Fluor-conjugated IgG (H+L) (1:1,000) in the dark at 37°C for 1 h. Cellular nuclei were counterstained with nuclear dye 4′,6-diamidino-2-phenylindole (DAPI) (1:2,000 diluted in PBS; Beyotime) for 5 min. Cells were observed under either inverted fluorescence microscope (Olympus Corporation, Tokyo, Japan) or confocal laser scanning microscope IX81-FV1000 (Olympus). Images were generated using FluoView FV1000 software version 3.1.2.2 (Olympus).
Nuclear dye 4 6 diamidino 2 phenylindole dapi
Manufactured by Beyotime
Sourced in China
DAPI is a fluorescent dye that binds to the minor groove of DNA, providing a simple and effective way to stain and visualize nucleic acids. It has excitation and emission wavelengths of 358 nm and 461 nm, respectively, allowing it to be detected using standard fluorescence microscopy techniques.
Automatically generated - may contain errors
Lab products found in correlation
Fv1000 ix81, by Olympus (1 mentions)
Inverted fluorescence microscope, by Olympus (1 mentions)
Fluoview fv1000, by Olympus (1 mentions)
Propidium iodide, by Dojindo Laboratories (1 mentions)
Cell counting kit 8 cck 8, by MedChemExpress (1 mentions)
1 3 diphenylisobenzofuran dpbf, by Merck Group (1 mentions)
Ammonium bicarbonate nh4hco3, by Merck Group (1 mentions)
Calcein am cam, by Dojindo Laboratories (1 mentions)
Singlet oxygen sensor green (sosg), by Thermo Fisher Scientific (1 mentions)
Calcium chloride dihydrate cacl2 2h2o, by Merck Group (1 mentions)
2 protocols using nuclear dye 4 6 diamidino 2 phenylindole dapi
1
Immunofluorescence Staining Protocol for Cultured Cells
2
Lipid-based Photosensitizer Delivery
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Ethanol (anhydrous, >99.7) and chloroform were obtained from Chongqing Chuandong Chemical Co. Ltd. (Chongqing, China). Calcium chloride dihydrate (CaCl2·2H2O), ammonium bicarbonate (NH4HCO3) and 1.3-diphenylisobenzofuran (DPBF) were purchased from Sigma Aldrich (MO, USA). HMME was purchased from Shanghai D&B Biotechnology Co., Ltd. (Shanghai, China). Nuclear dye 4′,6-Diamidino-2-phenylindole (DAPI) was purchased from Beyotime Biotechnology (Shanghai, China). Cell Counting Kit-8 (CCK-8) was bought from MedChemExpress (New Jersey, USA). Calcein-AM (CAM) and propidium iodide (PI) were obtained from Dojindo (Japan). Proliferating cell nuclear antigen (PCNA) antibody was obtained from Servicebio Technology Co., Ltd (Wuhan, China). Singlet oxygen sensor green (SOSG) was purchased from Thermo Fisher Scientific (California, USA). 1.2-dioleoyl-sn-glycerol-3-phosphate (sodium salt) (DOPA) was purchased from Avanti Polar Lipids, Inc. (Alabaster, Alabama, USA).1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000 (DSPE-PEG2000), 1.2-dipalmitoyl-sn-glycero-3-phospha-tidylcholine (DPPC) and cholesterol were synthesized by Xi’an Ruixi Biological Technology Co., Ltd (Xi’an, China).
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