Es 2030 freeze dryer

Manufactured by Hitachi

Sourced in Japan

The ES-2030 freeze dryer by Hitachi is a laboratory equipment designed for lyophilization, a process that removes water from a sample through sublimation. It features a condenser temperature of -85°C and a drying chamber volume of 30 liters.

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Lab products found in correlation

S 4800 field emission sem, by Hitachi (3 mentions) Ht7700 tem, by Hitachi (2 mentions) Glutaraldehyde solution, by TAAB (2 mentions) Lmw ta, by Electron Microscopy Sciences (1 mentions) Pelco colloidal graphite, by Ted Pella (1 mentions) S 4800 scanning electron microscope, by Hitachi (1 mentions) Sigma scanning electron microscope, by Zeiss (1 mentions) H 7500, by Hitachi (1 mentions) S 4700 sem, by Hitachi (1 mentions) Matrigel, by Corning (1 mentions) Glutaraldehyde, by Fujifilm (1 mentions) E 1030, by Hitachi (1 mentions) Jsm 7100f, by JEOL (1 mentions) T butyl alcohol, by Fujifilm (1 mentions)

Spelling variants of this product

ES-2030 (51 citations) E-2030 (2 citations)

10 protocols using es 2030 freeze dryer

Kidney Podocyte Ultrastructural Analysis by SEM

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Conventional SEM was performed as described previously (Dong et al., 2010 (link); Miyaki et al., 2020a (link)). Small cubes of the fixed kidney cortex (approximately 4 × 4 × 2 mm) were processed with conductive staining. First, the cubes were immersed in 1% OsO₄ in 0.1 M PB for 30 min at 24°C, washed with 0.1 M PB for 5 min three times, and then immersed in 1% LMW-TA (Electron Microscopy Sciences) in distilled water (DW) for 2 h at RT. After the cubes were washed three times with DW for 5 min, the same staining procedure was repeated twice. However, OsO₄ was diluted with DW.
The stained samples were dehydrated with a graded series of ethanol and then immersed in t-butyl alcohol. The samples were freeze-dried using an ES-2030 freeze dryer (Hitachi High-Technologies, Tokyo, Japan). The dried specimens were mounted on aluminum stubs with carbon paste (Pelco Colloidal Graphite, Ted Pella, Inc., Redding, CA, United States). The mounted specimens were coated with osmium with an OPC80T osmium plasma coater (Filgen, Inc., Nagoya, Japan). The samples were observed with an S-4800 field emission-SEM (Hitachi High-Technologies). Regions of interest were imaged using a backscattered electron detector with an acceleration voltage of 3 kV. Individual podocytes were denoted with different transparent colors using Adobe Illustrator.

Kawasaki Y., Hosoyamada Y., Miyaki T., Yamaguchi J., Kakuta S., Sakai T, & Ichimura K. (2021). Three-Dimensional Architecture of Glomerular Endothelial Cells Revealed by FIB-SEM Tomography. Frontiers in Cell and Developmental Biology, 9, 653472.

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Scanning Electron Microscopy of Kidney Cortex

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Small cubes of fixed kidney cortex were immersed in 2% osmium tetroxide in 0.1 M phosphate buffer for 2 hr. After dehydration with graded series of ethanol, specimens were transferred to t-butyl alcohol, and freeze-dried with an ES-2030 freeze dryer (Hitachi High-Technologies, Tokyo, Japan). After mounting on aluminum stubs with carbon paste, the dried specimens were coated with osmium with an OPC80T osmium plasma coater (Filgen, Nagoya, Japan), and observed with an S-4800 scanning electron microscope (Hitachi High-Technologies). Some fixed specimens were processed by alkaline maceration according to the previous method with some modifications19 (link)34 (link)35 (link). Briefly, the small cubes of fixed kidney cortex were placed in 6 M sodium hydrate for five min at 60°C, and then for 10 min at 45°C. After alkaline maceration, the tissues were rinsed in 0.01 M phosphate buffer (pH 7.3) containing 0.05% Tween 20 overnight. The macerated tissues were transferred into 50% aqueous solution of dimethyl sulfoxide, and then freeze-cracked in liquid nitrogen. The cracked samples were processed as were in conventional SEM protocol as described above.

Ichimura K., Miyazaki N., Sadayama S., Murata K., Koike M., Nakamura K.I., Ohta K, & Sakai T. (2015). Three-dimensional architecture of podocytes revealed by block-face scanning electron microscopy. Scientific Reports, 5, 8993.

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Collagen Fibril Formation Analysis

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Samples for SEM analysis at the three conditions (Collagen only, presence of OMD and E284R/E303R) were prepared and measured at the same time according to ref.^{11 (link)}. At first, fibril formation was carried out with the same method as TEM analysis. The fibril formed samples were adsorbed on super smooth silicon wafer (EM Japan) for 5 min followed by fixation with 1% glutaraldehyde in 0.1 M phosphate buffer for an hour at room temperature. The specimens were washed by phosphate buffer three times and post fixed with 1% OsO₄ in phosphate buffer for an hour on ice. They were dehydrated with a graded series of ethanol followed by t-butanol replacement. Then, samples were freeze dried using ES-2030 freeze dryer (Hitachi High-Technologies) and coated with OsO₄ using HPC-1S osmium coater (Vacuum Device). SEM was performed with Zeiss SIGMA scanning electron microscope.

Tashima T., Nagatoishi S., Caaveiro J.M., Nakakido M., Sagara H., Kusano-Arai O., Iwanari H., Mimuro H., Hamakubo T., Ohnuma S.I, & Tsumoto K. (2018). Molecular basis for governing the morphology of type-I collagen fibrils by Osteomodulin. Communications Biology, 1, 33.

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Conventional SEM Technique for Specimen Analysis

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Conventional SEM was performed as previously described (Dong et al. 2010) . In brief, fixed samples were immersed in 2% osmium tetroxide in 0.1 M phosphate buffer for 2 h. After dehydration using a graded series of ethanol, specimens were transferred to t-butyl alcohol and freeze-dried with an ES-2030 freeze dryer (Hitachi High-Technologies, Tokyo, Japan). After mounting on aluminum stubs with carbon paste, the dried specimens were coated with osmium using an OPC80T osmium plasma coater (Filgen, Nagoya, Japan) and observed using an S-4800 field-emission SEM (Hitachi High-Technologies).

Kawasaki Y., Matsumoto A., Miyaki T., Kinoshita M., Kakuta S., Sakai T, & Ichimura K. (2019). Three-dimensional architecture of pericardial nephrocytes in Drosophila melanogaster revealed by FIB/SEM tomography. Cell and tissue research, 378(2).

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Ultrastructural Analysis of Brain Regions

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Brains were fixed with 2% glutaraldehyde and 2% paraformaldehyde in 0.1 M phosphate buffer (PB; pH 7.4) for 1 hour, and then the ventral region of the brain, the septum, and the hippocampus were removed and fixed overnight at 4°C in the same solution. Samples were washed with 0.1 M PB 3 times on ice at 5-min intervals and postfixed with 1% osmium tetroxide in 0.1 M PB for 2 hours. The samples were then washed with distilled water 5 times on ice at 5-min intervals, and dehydrated 3 times in an ethanol series. The ethanol was cleared with tert-butyl alcohol, and the samples were freeze-dried (ES-2030 Freeze Dryer; Hitachi), vapor-deposited with an HPC-1S osmium coater (Vacuum Devices) and examined with a field-emission scanning electron microscope (Model S4500; Hitachi).

Zheng H., Yu W.M., Waclaw R.R., Kontaridis M.I., Neel B.G, & Qu C.K. (2018). Gain-of-function mutations in the protein tyrosine phosphatase Ptpn11 (SHP2) induce hydrocephalus in a catalytically-dependent manner. Science signaling, 11(522), eaao1591.

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Lung Tissue Ultrastructural Analysis

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Using a scanning electron microscope (SEM), lung tissues resected during surgery for SP (and cultured PMCs) were fixed with 2% glutaraldehyde solution (TAAB Laboratories Equipment Ltd., Berks, England) in 0.1 M phosphate buffer (pH 7.4), followed by postfixation with 2% OsO₄ in the same buffer. Fixed specimens were dehydrated with a graded series of ethanol. Dehydrated specimens were transferred into t-butyl alcohol and freeze-dried with an ES-2030 freeze dryer (Hitachi, Tokyo, Japan). After mounting on aluminum stubs with carbon paste, the dried specimens were coated with osmium using an OPC80T osmium plasma coater (Filgen, Inc., Aichi, Japan) and observed with an S-4800 field-emission SEM (Hitachi).
Using a transmission electron microscope (TEM), resected lung tissues were fixed with 2.5% glutaraldehyde, followed by the same postfixation procedure described above. Fixed specimens were dehydrated and embedded in Epok812 (Okenshoji Co., Ltd., Tokyo, Japan). Ultrathin sections were cut and stained with uranyl acetate and lead citrate. These sections were examined with an HT7700 TEM (Hitachi). Reproducibility was confirmed in 3 unrelated samples per group.

Okamoto S., Ebana H., Kurihara M., Mitani K., Kobayashi E., Hayashi T., Sekimoto Y., Nishino K., Otsuji M., Kumasaka T., Takahashi K, & Seyama K. (2021). Folliculin haploinsufficiency causes cellular dysfunction of pleural mesothelial cells. Scientific Reports, 11, 10814.

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Ultrastructural Analysis of Lung Tissues

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Using a scanning electron microscope (SEM), lung tissues resected during surgery for SP (and cultured PMCs) were xed with 2% glutaraldehyde solution (TAAB Laboratories Equipment Ltd., Berks, England) in 0.1 M phosphate buffer (pH 7.4), followed by post xation with 2% OsO 4 in the same buffer. Fixed specimens were dehydrated with a graded series of ethanol. Dehydrated specimens were transferred into t-butyl alcohol and freeze-dried with an ES-2030 freeze dryer (Hitachi, Tokyo, Japan). After mounting on aluminum stubs with carbon paste, the dried specimens were coated with osmium using an OPC80T osmium plasma coater (Filgen, Inc., Aichi, Japan) and observed with an S-4800 eld-emission SEM (Hitachi).
Using a transmission electron microscope (TEM), resected lung tissues were xed with 2.5% glutaraldehyde, followed by the same post xation procedure described above. Fixed specimens were dehydrated and embedded in Epok812 (Okenshoji Co., Ltd., Tokyo, Japan). Ultrathin sections were cut and stained with uranyl acetate and lead citrate. These sections were examined with an HT7700 TEM (Hitachi). Reproducibility was con rmed in 3 unrelated samples per group.

Okamoto S., Ebana H., Kurihara M., Mitani K., Kobayashi E., Hayashi T., Sekimoto Y., Nishino K., Otsuji M., Kumasaka T., Takahashi K., & Seyama K. (2021). Folliculin Haploinsufficiency Causes Cellular Dysfunction of Pleural Mesothelial Cells.

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Microstructural Analysis of Termite Species

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We studied three species: Embiratermes neotenicus (Termitidae) collected from Petit Saut, French Guiana; Hodotermopsis sjostedti (Termopsidae) from Yakushima Island, Kagoshima Prefecture, Japan; Nasutitermes takasagoensis (Termitidae) from Yaeyama Islands, Okinawa Prefecture, Japan. In preparation for observations by microCT (Phoenix Nanotom-180; PhoenixjX-ray, GE Sensing & Inspection Technologies, Wunstorf, Germany), specimens were fixed in either Bouins´s solution or 70% ethanol. Specimens were dehydrated in an ethanol series, freezedried with ES-2030 freeze dryer (Hitachi, Tokyo). Three-dimensional reconstructions were performed using Imaris 6.4.0 (Bitplane AG, Switzerland), and WinSURF (SURFdriver Software, Kailua, HI). Reconstructed images for figures were arranged with Adobe Photoshop CS5. For transmission electron microscopy (TEM; Hitachi H-7500, Tokyo, Japan), living specimens were initially fixed in 2% glutaraldehyde and 2% paraformaldehyde in 0.1 M cacodylate sodium buffer (pH 7.4) for 5 h at 4°C; postfixed in 1% osmium tetroxide in the same buffer with 5% sucrose for 2 h at 4°C; dehydrated through a graded acetone series; and then embedded in Spurr's resin and polymerized. Sections were obtained using an ultramicrotome and stained with a 1% potassium permanganate solution in distilled water for 2 min followed by 2% lead citrate for 3 min.

Kaji T., Keiler J., Bourguignon T, & Miura T. (2016). Functional transformation series and the evolutionary origin of novel forms: evidence from a remarkable termite defensive organ. Evolution & development, 18(2).

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Beef Sample Contamination and Disinfection

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We prepared beef samples contaminated with the EDL933 strain treated with or without high-speed washing in the CEL disinfectant for 5 min. We submitted these samples to the Graduate School of Medicine at Kyoto University to obtain scanning electron microscope (SEM) photos. The specimens were fixed with 4% paraformaldehyde and 0.05% glutaraldehyde at 4°C for 4 h. After post-fixation with 1% OsO₄ for 2 h, they were dehydrated using Freeze Dryer ES-2030 (Hitachi Ltd., Tokyo, Japan) and coated with a thin layer of platinum palladium using Ion Coater IB3 (Eiko Corporation, Tokyo, Japan). The specimens were examined with a Hitachi S-4700 SEM (Hitachi Ltd., Tokyo, Japan).

Kayali A.Y., Ozawa J, & Nishibuchi M. (2020). Development and Improvement of Methods to Disinfect Raw Beef Using Calcium Hydroxide–Ethanol–Lactate-Based Food Disinfectant for Safe Consumption. Frontiers in Microbiology, 11, 537889.

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SDSE Strain Preparation for SEM

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Scanning Electron Microscopy analyses were performed as described previously with some modifications (Honda-Ogawa et al., 2017 (link)). SDSE strains were grown overnight in THY medium or RPMI 1640 supplemented with 10% fresh human serum at 37°C under 5% CO₂ atmosphere. Bacterial cells were fixed with 2.5% glutaraldehyde (FUJIFILM Wako Pure Chemical Corporation) on aluminum plates coated with Matrigel (Corning, Inc., NY, United States) at room temperature for 1 h and washed twice with PBS. After serial dehydration with 50, 80, and 100% ethanol, the samples were soaked in 100% t-butyl alcohol (FUJIFILM Wako Pure Chemical Corporation), freeze-dried (Freeze Dryer ES-2030, Hitachi Technologies, Tokyo Japan), and coated with 60% Au-Pd alloy (Hitachi E-1030, Hitachi Technologies; sputtering conditions: 15 mA, 7 Pa, 30 s). The SEM images were obtained using JSM-7100F (JEOL Ltd., Tokyo, Japan).

Matsue M., Ogura K., Sugiyama H., Miyoshi-Akiyama T., Takemori-Sakai Y., Iwata Y., Wada T, & Okamoto S. (2020). Pathogenicity Characterization of Prevalent-Type Streptococcus dysgalactiae subsp. equisimilis Strains. Frontiers in Microbiology, 11, 97.

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