Endopan 3 complete medium for ecs

Manufactured by PAN Biotech

Endopan 3 is a complete medium formulated for the cultivation of endothelial cells (ECs). It provides the necessary nutrients and growth factors to support the growth and proliferation of ECs in vitro.

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Lab products found in correlation

Streptomycin, by Thermo Fisher Scientific (2 mentions) Penicillin, by Thermo Fisher Scientific (2 mentions) Penicillin, by R&D Systems (1 mentions) Streptomycin, by R&D Systems (1 mentions) Hbmecs, by R&D Systems (1 mentions)

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Endopan 3 medium (10 citations) Endopan-3 (3 citations)

2 protocols using endopan 3 complete medium for ecs

Culturing Brain Endothelial Cells

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Human brain microvascular endothelial cells (HBMECs) (ACBRI 376, Cell Systems) were cultured in well plates coated with 0.1% gelatin in Endopan 3 complete medium for ECs (P04-0010K, PAN-Biotech) supplemented with 10% FBS, 100 U ml⁻¹ of penicillin and 100 μg mL⁻¹ of streptomycin (15140122, ThermoFisher) in a 5% CO₂ humidified incubator at 37°C. HBMECs were used from passages 6 to 10 for the experiments.

Vieira J.R., Shah B., Dupraz S., Paredes I., Himmels P., Schermann G., Adler H., Motta A., Gärtner L., Navarro-Aragall A., Ioannou E., Dyukova E., Bonnavion R., Fischer A., Bonanomi D., Bradke F., Ruhrberg C, & Ruiz de Almodóvar C. (2022). Endothelial PlexinD1 signaling instructs spinal cord vascularization and motor neuron development. Neuron, 110(24), 4074-4089.e6.

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Culturing and Stimulating Brain Endothelial Cells

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HBMECs (Cell Systems) and primary SC ECs sorted after MACS enrichment were cultured in well plates coated with 0.1% gelatine in Endopan 3 complete medium for ECs (PAN-Biotech) supplemented with 10% FBS, 100 U ml⁻¹ of penicillin and 100 μg ml⁻¹ of streptomycin (both Gibco by Life Technologies) in a 5% CO₂ humidified incubator at 37 °C. HBMECs from passages 6 to 10 were used for the experiments. HBMECs and primary SC ECs were starved in growth factor-free Endopan 3 supplemented with 2% FBS, 100 U ml⁻¹ of penicillin and 100 μg ml⁻¹ of streptomycin before stimulation with recombinant Ang1 300 ng ml⁻¹ (923-AN, R&D systems), Ly294002 (440202, Calbiochem) 40 μM or vehicle. After 24 h, cells were lysed for RNA extraction.

Paredes I., Vieira J.R., Shah B., Ramunno C.F., Dyckow J., Adler H., Richter M., Schermann G., Giannakouri E., Schirmer L., Augustin H.G, & de Almodóvar C.R. (2021). Oligodendrocyte precursor cell specification is regulated by bidirectional neural progenitor–endothelial cell crosstalk. Nature neuroscience, 24(4), 478-488.

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