Anti cd1c pecy7

ErcDCs and macrophages were sorted from ccRCC tissue-cell-suspensions stained with CD45-PeCy7, CD11c-APC, CD3-PB, CD209-PE (all BD Biosciences), CD14-PerCPCy5.5 (eBioscience, San Diego, CA, USA) and LIVE/DEAD^® Fixable Near-IR Dead Cell Stain Kit (Thermo Fisher Scientific). Sorting gates were set on CD209⁺CD14⁺ cells (ercDCs) and CD209⁻CD14⁺ cells (macrophages), among pre-gated CD45⁺, live, single CD11c⁺ CD3⁻ cells. CD1c⁺ DC and slanDCs were sorted from B- and NK-depleted PBMCs of healthy donors (HD) using anti-CD11c-PE, anti-CD3-PB (all BD Biosciences), anti-CD56-APC (Beckman Coulter, Brea, CA, USA), anti-CD19-PB (Dako by Agilent, Santa Clara, CA, USA), anti-CD1c-PeCy7 (Biolegend, San Diego, CA, USA), anti-slan-FITC (Miltenyi Biotec) and LIVE/DEAD^® Fixable Near-IR Dead Cell Stain Kit. The gating strategy and instrument parameters are in Supplemental Figure S1. Gates were set very strictly, not covering the whole population, to avoid contamination with other cell populations. Cell population purity varied between 98–100%. Cells were directly sorted into 250 µL of RLT lysis buffer with ß-mercaptoethanol (RNeasy Micro Kit by Qiagen, Venlo, The Netherlands) using FACSAria IIIu (BD Biosciences), then homogenized (QIAshredder by Qiagen) and stored at −80 °C. Details about sorted cell types and numbers of biological replicates are listed in Table S2.