Fontana masson stain kit

Manufactured by Merck Group

The Fontana-Masson stain kit is a laboratory reagent used to detect the presence of melanin pigment in tissue samples. It provides a reliable and effective method for the identification and visualization of melanin-containing cells, such as melanocytes, in histological preparations.

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Lab products found in correlation

Envision system hrp, by Agilent Technologies (1 mentions) Trp 2, by Abcam (1 mentions) Keratin 6, by Fortrea (1 mentions) Keratin 10, by Fortrea (1 mentions) Axiovert 35, by Zeiss (1 mentions)

Spelling variants of this product

Masson) kit (8 citations) Masson Stain Kit (2 citations)

2 protocols using fontana masson stain kit

Histological Analysis of Skin Tissue

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Fixed skin tissues were embedded in paraffin and sectioned. The sections were stained with hematoxylin and eosin, hematoxylin alone, or with hematoxylin combined with the following antibodies: BrdU, total p53, and γ-H2AX (14 (link)); keratin-6 (rabbit polyclonal, 1:500; Covance); keratin-10 (rabbit polyclonal, 1:500; Covance); or TRP2 (rabbit polyclonal, 1:500; Abcam). The tissues were then labeled with secondary antibodies.EnVision+ System HRP labeled polymer; anti-rabbit HRP (Dako) was used for the rabbit polyclonal antibodies. Melanin was stained with a Fontana-Masson stain kit (Sigma-Aldrich procedure no.: HT200). Stained slides were digitally scanned using a ScanScope CS (Aperio).

Lange S.S., Bhetawal S., Reh S., Powell K.L., Kusewitt D.F, & Wood R.D. (2018). DNA polymerase ζ deficiency causes impaired wound healing and stress-induced skin pigmentation. Life Science Alliance, 1(3), e201800048.

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Histological Characterization of Skin Tissue

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The reconstructed and ex vivo skin tissue were fixed in 4% paraformaldehyde solution and processed for embedding in paraffin blocks. The blocks were cut in 5 μm sections and stained with hematoxylin and eosin (H&E). Skin biopsy cuts were also stained for Fontana Masson using the Sigma-Aldrich Fontana Masson stain kit, Masson’s Trichrome staining, and Picrosirius Red staining for collagen fibers. Pictures were acquired with a LEICA DMLS Microscope (Leica Mikroskopie und Systeme, Wetzlar). Polarization images were recorded with a true color RGB camera (Spot insight, Diagnostic Instruments Inc., USA) on a Zeiss inverted microscope (Axiovert 35) equipped with a Zeiss LD Achroplan 40 × long distance objective, NA 0.6. Crossed polarizers were placed between the condenser (0.55 NA; DIC 0.3-0.4 position) and the rear aperture of the objective. Polychromatic white light (3200 K; 100 W halogen lamp) was used throughout.

Wedel S., Martic I., Hrapovic N., Fabre S., Madreiter-Sokolowski C.T., Haller T., Pierer G., Ploner C., Jansen-Dürr P, & Cavinato M. (2020). tBHP treatment as a model for cellular senescence and pollution-induced skin aging. Mechanisms of ageing and development, 190, 111318.

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