Single-cell suspensions of cells were washed three times in PBS containing 1% BSA followed by incubation in 100 µl PBS/1% BSA containing anti-CD24 (PE) and anti-CD44 (FITC) antibody (BD Biosciences) for 2 hours at room temperature. Cells were then washed three times in PBS containing 1% BSA and resuspended in 500 µl PBS. Samples were analyzed using the BD LSRFortessa Analytic Flow Cytometer. FACS sorting of CD44+/CD24− and CD44−/CD24+ HMLE populations was performed using the FACSAria II Cell Sorter and FACSDiva™ 6 software (BD Biosciences).
Anti cd24 pe and anti cd44 fitc antibody
Manufactured by BD
The Anti-CD24 (PE) and Anti-CD44 (FITC) Antibody is a flow cytometry reagent used for the detection and quantification of CD24 and CD44 expression on cells. CD24 is a cell surface glycoprotein, and CD44 is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion, and migration. This antibody can be used in research applications to identify and characterize cell populations expressing these markers.
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2 protocols using anti cd24 pe and anti cd44 fitc antibody
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Flow Cytometry for CD44/CD24 Profiling
2
Flow Cytometry for CD44/CD24 Profiling
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Single-cell suspensions of cells were washed three times in PBS containing 1% BSA followed by incubation in 100 µl PBS/1% BSA containing anti-CD24 (PE) and anti-CD44 (FITC) antibody (BD Biosciences) for 2 hours at room temperature. Cells were then washed three times in PBS containing 1% BSA and resuspended in 500 µl PBS. Samples were analyzed using the BD LSRFortessa Analytic Flow Cytometer. FACS sorting of CD44+/CD24− and CD44−/CD24+ HMLE populations was performed using the FACSAria II Cell Sorter and FACSDiva™ 6 software (BD Biosciences).
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