Methanol and dimethyl sulfoxide dmso

TMB, dopamine (DOPA) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were purchased from Sigma-Aldrich (Steinheim, Germany), and methanol and dimethyl sulfoxide (DMSO) were purchased from Merck (Shanghai, China). All of the other chemicals were of analytical grade and purchased from Damao (Tianjin, China) or Aladdin (Shanghai, China). The metal salts used in this study were all in the chloride form, and the anions were all in the sodium form.

1,2-distearoyl-sn-glycero-3 phosphoethanolamine-N-[methoxy (polyethylene glycol) - 2000] amine (DSPE-PEG-NH₂) and dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) were purchased from Avanti polar lipid (AL, USA). ICG was purchased from BioActs (Korea). Branched polyethyleneimine (1800 Da) was purchased from Polysciences Inc. (USA). Methanol and dimethyl sulfoxide (DMSO) were obtained from Merck (Germany). 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC), L-glutathione reduced (GSH), N-hydroxysuccinimide (NHS), lithocholic acid and L-buthionine sulfoximine (BSO) were purchased from Sigma (St. Louis, MO). 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4sulfophenyl)- tetrazolium (MTS) reagent was obtained from Promega (Madison, WI, USA). Propidium iodide (PI), fluorescein diacetate (FDA) and Dulbecco's modified Eagle's medium (DMEM) Ellman's reagent were obtained from Thermo Scientific (Waltham, MA, USA). All other chemicals were obtained from commercial suppliers unless otherwise specified.