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Staphylococcus haemolyticus

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Staphylococcus haemolyticus is a Gram-positive, coagulase-negative bacterium. It is a common inhabitant of human skin and mucous membranes. Staphylococcus haemolyticus is known to produce beta-hemolysis on blood agar.

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Lab products found in correlation

Pseudomonas aeruginosa, by American Type Culture Collection (4 mentions) Enterococcus faecalis, by American Type Culture Collection (4 mentions) Staphylococcus epidermidis, by American Type Culture Collection (3 mentions) Streptococcus pyogenes, by American Type Culture Collection (3 mentions) Escherichia coli, by American Type Culture Collection (2 mentions) Bacillus cereus, by American Type Culture Collection (2 mentions) Klebsiella pneumonia, by American Type Culture Collection (2 mentions) Proteus mirabilis, by American Type Culture Collection (2 mentions) Nanodrop 2000 spectrophotometer, by Thermo Fisher Scientific (2 mentions) Acinetobacter junii, by American Type Culture Collection (1 mentions) Streptococcus iniae, by American Type Culture Collection (1 mentions) Type 1 agar, by Merck Group (1 mentions) Aeromonas hydrophilia, by American Type Culture Collection (1 mentions) Salmonella typhimurium, by American Type Culture Collection (1 mentions) Bacillus subtilis, by American Type Culture Collection (1 mentions) S agalactiae, by American Type Culture Collection (1 mentions) E faecalis, by American Type Culture Collection (1 mentions) Enterobacter aerogenes, by American Type Culture Collection (1 mentions) S pneumonia, by American Type Culture Collection (1 mentions) Staphylococcus aureus, by American Type Culture Collection (1 mentions)

4 protocols using staphylococcus haemolyticus

1

Antimicrobial Activity of Cathelicidin-OA1

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The antimicrobial activity of cathelicidin-OA1 was assayed based on our previous research31 (link). Briefly, gram-positive bacterial strains Staphylococcus epidermidis (ATCC 12228), Staphylococcus haemolyticus (ATCC 29970), and Enterococcus faecalis (ATCC 29212), gram-negative bacterial strains Escherichia coli (ATCC 25922), Salmonella paratyphi A (ATCC 9150), Pseudomonas aeruginosa (ATCC 27853), Acinetobacter junii (ATCC 17908), Aeromonas hydrophilia (ATCC 49140, Vibrio splendidus (ATCC 33869), and Streptococcus iniae (ATCC 29177), and fungal strains Candida glabrata (ATCC 66032) and Candida albicans (ATCC 14053) were obtained from the Kunming Medical University. The microbes were grown in Luria Bertani (LB) broth to OD600 = 0.8. A 10 μl aliquot of each bacterium was collected and added to 10 ml of fresh LB broth with 1% Type I agar (Sigma-Aldrich, St Louis, MO, USA), then poured over a 90-mm Petri dish. When the agar hardened, a small hole was made and a 7 μl aliquot of cathelicidin-OA1 (1 mM) was added to the hole, followed by incubation for 16–18 h at 37 °C. If the sample contained antimicrobial activity, a clear zone appeared on the surface of the agar, representing inhibition of bacterial growth.
Cao X., Wang Y., Wu C., Li X., Fu Z., Yang M., Bian W., Wang S., Song Y., Tang J, & Yang X. (2018). Cathelicidin-OA1, a novel antioxidant peptide identified from an amphibian, accelerates skin wound healing. Scientific Reports, 8, 943.
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2

Antibiotic Susceptibility of Bacterial Strains

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For this study, seventeen type strains: Staphylococcus aureus (ATCC 29737), S. aureus (NCTC 6571), Streptococcus pyogenes (ATCC 12344), S. pneumonia (ATCC 10015), S. agalactiae (ATCC12386), Enterococcus faecalis (ATCC 19433), E. faecalis (ATCC 49532), Salmonella typhimurium (ATCC 14028), Pseudomonas aeruginosa (ATCC 10145), Staphylococcus haemolyticus (ATCC 29970), Proteus mirabilis (NCIMB 13283), Enterobacter Aerogenes (ATCC13048), E. aerogenes (NCIMB 10102), Bacillus subtilis (ATCC 11774), Klebsiella pneumonia (ATCC 9633), Bacillus cereus (ATCC 10876) and Staphylococcus epidermidis (ATCC 12228) were examined. In addition, 10 clinical isolates of S. aureus, S. typhimurium, E. coli, S. pneumonia and K. pneumonia were also used. To verify the accuracy of the susceptibility results, strains of Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 29213) and Enterococcus faecalis (ATCC 29212) were used as control organisms.
Anwer M.K., Iqbal M., Muharram M.M., Mohammad M., Ezzeldin E., Aldawsari M.F., Alalaiwe A, & Imam F. (2020). Development of Lipomer Nanoparticles for the Enhancement of Drug Release, Anti-Microbial Activity and Bioavailability of Delafloxacin. Pharmaceutics, 12(3), 252.
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3

Bacterial Strain Identification Protocol

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A total of 16 reference bacterial strains of S. aureus (ATCC-25923), Staphylococcus hominis (ATCC-27844), Staphylococcus haemolyticus (ATCC-29970), Staphylococcus epidermidis (ATCC-12228), Staphylococcus saprophyticus (ATCC-15305), Streptococcus pneumoniae (ATCC-49619), Streptococcus agalactiae (ATCC-13813), Streptococcus pyogenes (ATCC-19615), Enterococcus faecalis (ATCC-49149), K. pneumonia (ATCC-13883), Proteus mirabilis (ATCC-35659), Pseudomonas aeruginosa (ATCC-27853), Escherichia coli (ATCC-25922), Bacillus cereus (ATCC-14579), Salmonella enterica (ATCC-14028), and Listeria monocytogenes (ATCC-7644) were purchased from BIOBW (China). Eight AMR S. aureus strains were acquired from the College of Biomedicine and Health, Huazhong Agricultural University, China. Culture-based biochemical and susceptibility tests, whole-genome sequencing (WGS), and PCR-Sanger sequencing were used to identify all bacterial strains. Following the manufacturer's directions, genomic DNA (gDNA) was isolated from bacterial cells collected from the bacterial culture medium using a Bacteria Genomic DNA Extraction Kit (TIANamp Biotechnology Co., Ltd., Beijing, China). All extracted gDNA was quantified using a NanoDrop2000 spectrophotometer (Thermo Fisher Scientific UK, no longer available) and kept at –20°C until testing.
Lin C., Zeng Y., Zhu Z., Liao J., Yang T., Liu Y., Wei H., Li J., Ma J., Wu X., Lin G., Lin L., Chen L., Huang H., Chen W., Wang J., Wen F, & Lin M. (2023). A Rapid Antimicrobial Resistance Diagnostic Platform for Staphylococcus aureus Using Recombinase Polymerase Amplification. Microbiology Spectrum, 11(2), e04476-22.
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4

Bacterial Species Identification Protocol

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Bacterial species E. coli ATCC 25922 used for the test. For cross reaction these bacteria were used: Klebsiella pneumonia ATCC 70603, Staphylococcus haemolyticus PTCC 1437, Streptococcus pyogenes PTCC 1447, Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 27863, Proteus mirabilis ATCC 25933, Serratia marcescens ATCC 13880, E. coli PTCC1270, E. coli PTCC 1276. All the bacteria were obtained from Iranian Research Organization for Science and Technology (IROST). The bacteria were subcultured in Tryptic Soy Broth (TSB) and bacterial stocks were prepared by 70% glycerol. Bacteria were subcultured in Luria broth (LB) at 37 °C overnight, until it gets the OD (600) of 1 that is approximately 5 × 108 to 1 × 109 cells/ml4 (link).
Hashemi E, & Forouzandeh M. (2018). Designing a new biosensor “DNA ELISA” to detect Escherichia coli using genomic DNA and comparison of this method to PCR-ELISA. Journal of Enzyme Inhibition and Medicinal Chemistry, 33(1), 722-725.
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