Isopropyl 1 thio beta d galactopyranoside

Manufactured by Qiagen

Sourced in United States

Isopropyl-1-thio-beta-D-galactopyranoside is a synthetic compound used in molecular biology as an inducer for gene expression in bacteria. It is a structural analog of lactose and functions by binding to and inactivating the lac repressor protein, allowing for the expression of genes under the control of the lac operon. This product is commonly used in research applications involving recombinant protein production.

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Lab products found in correlation

Pet22b, by Merck Group (2 mentions) Pet21b, by Merck Group (2 mentions) Ni nta agarose, by Qiagen (2 mentions) Carbenicillin, by Merck Group (2 mentions) Luria bertani medium, by Merck Group (2 mentions) Horseradish peroxidase conjugated anti human igg or iga antibodies, by Agilent Technologies (2 mentions)

2 protocols using isopropyl 1 thio beta d galactopyranoside

Recombinant Mycobacterial Antigen Production

Cited 1 time

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pET-21b, pET-22b, Luria-Bertani (LB) medium and carbenicillin were obtained from Sigma (St. Louis, MO, USA); isopropyl-1-thio-beta-D- galactopyranoside and Ni-NTA agarose were obtained from Qiagen (Gaithersburg, MD, USA); skim milk was from Morinaga (Tokyo, Japan); horseradish peroxidase-conjugated anti-human IgG or IgA antibodies was from Dako (Carpinteria, CA, USA); and SureBlue reserve TMB microwell peroxidase substrate was obtained from KPL (Gaithersburg, MD, USA). Expression and purification procedures for recombinant mycobacterial antigens (ESAT-6, CFP10, MDP1, Ag85A, Acr, HBHA and HrpA) were described previously [34 (link)].

Niki M., Yoshiyama T., Nagai H., Miyamoto Y., Niki M., Oinuma K.I., Tsubouchi T., Kaneko Y., Matsumoto S., Sasaki Y, & Hoshino Y. (2020). Nutritional status positively impacts humoral immunity against its Mycobacterium tuberculosis, disease progression, and vaccine development. PLoS ONE, 15(8), e0237062.

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Recombinant Mycobacterial Antigens Expression

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

pET-21b, pET-22b, Luria-Bertani (LB) medium, and carbenicillin were from Sigma (St. Louis, MO, USA); isopropyl-1-thio-beta-D- galactopyranoside and Ni-NTA agarose were from Qiagen (Gaithersburg, MD, USA); skim milk was from Morinaga (Tokyo, Japan); horseradish peroxidase-conjugated anti-human IgG or IgA antibodies were from Dako (Carpinteria, CA, USA); SureBlue reserve TMB microwell peroxidase substrate was from KPL (Gaithersburg, MD, USA). Expression and purification procedures for recombinant mycobacterial antigens (ESAT-6, CFP-10, MDP1, Ag85A, Acr, HBHA, and HrpA [Acr2]) were described previously [36 (link)].

Niki M., Yoshiyama T., Miyamoto Y., Okumura M., Niki M., Oinuma K.I., Kaneko Y., Matsumoto S., Sasaki Y., Ogata H., Goto H., Kudoh S, & Hoshino Y. (2018). Longitudinal Evaluation of Humoral Immunity and Bacterial and Clinical Parameters Reveals That Antigen-Specific Antibodies Suppress Inflammatory Responses in Active Tuberculosis Patients. Journal of Immunology Research, 2018, 4928757.

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