Hoechst staining kit

Berberine (BBR), evodiamine (EVO), coptisine (COP) (Fig. 1C), luteolin (LUT) (Fig. 1D), and cyanidin-3-O-glucoside (C3G) (Fig. 1E) were purchased from Chengdu Pufei De Biotech Co., Ltd (Chengdu, China). Tetraethyl orthosilicate (TEOS) was purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). DSPE-PEG2000, cholesterol, and hydrogenated lecithin (HSPC) were purchased from Shanghai Yuanye Biotechnology Co., Ltd (Shanghai, China). Sodium carbonate, ammonia, chloroform, and 95% ethanol were purchased from Chengdu Kelong Chemical Reagent Plant (Chengdu, China). Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and phosphate-buffer solution (PBS) were purchased from Gibco (Uxbridge, UK). Hoechst Staining Kit, Annexin V-FITC Apoptosis Detection Kit, trypsin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO). All other reagents were of analytical grade and were used as supplied.

Antibodies against IGF2BP1 (ab100999), phosphorylated p38 (p-p38) (ab31828), p-ERK (ab214362), p21 (p21), and cleaved caspase-3 (ab13847) were purchased from Abcam. IgG-HRP antibodies were purchased from Wuhan Boster Biological Technology, Ltd., (Wuhan, China) (#BA1054). Reverse Transcriptase kit (RT-PCR) was purchased from Takara. Total Protein Extraction kit was purchased from Thermo Fisher Scientific (#23227). Matrigel was obtained from Corning Inc., (Corning, NY, USA) (#354248). Hoechst staining kit was purchased from Sigma-Aldrich, St. Louis, MO, USA (#B2883). Cell-Light™ EdU Apollo^® 488/567 In Vitro Imaging kit was purchased from Guangzhou RiboBio Co., Ltd. (Guangzhou, China) (#C10327). PI/Annexin V-FITC apoptosis detection kit was purchased from MultiSciences Biotech Co., Ltd. (Hangzhou, China) (#CCS012).