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Gelbond pag film for acrylamide gels

Manufactured by Lonza
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Lonza GelBond PAG Film is a ready-to-use product for the preparation of polyacrylamide gels. It serves as a support film for the gel, facilitating its handling and transfer.

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Lab products found in correlation

Gelslick 50640, by Lonza (2 mentions) Bovine serum albumin bsa a9418, by Merck Group (2 mentions) Phosphate buffered saline ph 7, by Thermo Fisher Scientific (1 mentions) Triton x 100 bp 151, by Thermo Fisher Scientific (1 mentions) Ultrapure water system, by Merck Group (1 mentions)

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GelBond film (18 citations) GelBond (4 citations)

2 protocols using gelbond pag film for acrylamide gels

1

Biomaterial Surface Functionalization Protocol

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Tetramethylethylenediamine (TEMED; T9281), ammonium persulfate (APS; A3678), β-mercaptoethanol (M3148), 30%T/2.7%C acrylamide/bis-acrylamide (37.5:1) (A3699), bovine serum albumin (BSA; A9418), Tyrode’s solution (T1788), trypsin 10× (59427C), Accutase (A6964), and 3-(trimethoxylsilyl)propyl methacrylate (440159) were purchased from Sigma-Aldrich. Triton X-100 (BP-151) and phosphate-buffered saline (PBS; pH 7.4; 10010023) were purchased from Thermo Fisher Scientific. Premixed 10× tris-glycine EP (electrophoresis) buffer [25 mM tris (pH 8.3), 192 mM glycine] was purchased from Bio-Rad. Tris-buffered saline with Tween-20 (TBST) was prepared from 20× TBST (sc-24953, Santa Cruz Biotechnology). Deionized water (18.2 megohms) was obtained using an ultrapure water system from Millipore. Alexa 555–labeled BSA (A34786) was purchased from Invitrogen. N-[3-[(3-benzoylphenyl)formamido]propyl] methacrylamide (BPMAC) was custom synthesized by Pharm-Agra Laboratories (Brevard, NC). GelSlick (50640) and Lonza GelBond PAG Film for Acrylamide Gels (BMA54746) were purchased from Lonza.
Rosàs-Canyelles E., Modzelewski A.J., Geldert A., He L, & Herr A.E. (2020). Assessing heterogeneity among single embryos and single blastomeres using open microfluidic design. Science Advances, 6(17), eaay1751.
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2

Comprehensive Protein and DNA Analysis

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Tetramethylethylenediamine (TEMED, T9281), ammonium persulfate (APS, A3678), β-mercaptoethanol (M3148), 30%T/2.7%C acrylamide/bis-acrylamide (37.5:1) (A3699), bovine serum albumin (BSA, A9418), Tyrode’s solution (T1788), trypsin 10X (59427C), digitonin (D141), sucrose (S0389–500G), magnesium chloride (M8266) and HEPES (90909C) were purchased from Sigma-Aldrich. Triton X-100 (BP-151), phosphate-buffered saline (PBS, pH 7.4, 10010023), SYBR Gold (S11494), agarose (BP-1356–500) were purchased from Thermo Fisher Scientific. Premixed 10X tris-glycine electrophoresis buffer (25 mM Tris, pH 8.3; 192 mM glycine; 0.1% SDS) was purchased from Bio-Rad. Tris buffered saline with Tween-20 (TBST) was prepared from 20X TBST (sc-24953, Santa Cruz Biotechnology, Dallas, TX). Deionized water (18.2 MΩ) was obtained using an Ultrapure water system from Millipore. N-[3-[(3-Benzoylphenyl)formamido]propyl] methacrylamide (BPMAC) was custom synthesized by Pharm-Agra Laboratories (Brevard, NC). GelSlick™ (50640) and Lonza™ GelBond™ PAG Film for Acrylamide Gels (BMA54746) was purchased from Lonza. Taq PCR kit (E5000S), proteinase K (P8107S) were purchased from New England Biosciences.
Rosàs-Canyelles E., Modzelewski A.J., Martinez A.E., Geldert A., Gopal A., He L, & Herr A.E. (2021). Multimodal detection of protein isoforms and nucleic acids from low starting cell numbers. Lab on a chip, 21(12), 2427-2436.
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