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Cd27 alx700

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CD27-Alx700 is a fluorochrome-conjugated antibody that specifically binds to the CD27 cell surface receptor. CD27 is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on various immune cell types, including T cells, B cells, and natural killer cells. The Alx700 fluorochrome allows for the detection and analysis of CD27-expressing cells using flow cytometry.

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Lab products found in correlation

Facsaria, by BD (2 mentions) Cd123 pe cy5, by BD (2 mentions) Cd20 bv570, by BioLegend (2 mentions) Cd4 bv785, by BioLegend (2 mentions) Cd14 bv650, by BioLegend (2 mentions) Cd19 bv605, by BD (2 mentions) Cd8 pacblue, by Thermo Fisher Scientific (2 mentions) Tcrγδ apc, by BD (2 mentions) Cd16 percp cy5, by BioLegend (2 mentions) Cd32 pe, by BioLegend (2 mentions) Cd45ro ecd, by Beckman Coulter (2 mentions)

2 protocols using cd27 alx700

1

Isolation and Characterization of CD4+ T Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Participant recruitment and informed consent were performed under Institutional Review Board (IRB)-approved protocols at the US National Institutes of Health (NIH) and University of Washington. Peripheral blood mononuclear cells (PBMC) were isolated from whole blood by density gradient centrifugation. PBMC were incubated with Fcγ-receptor-blocking reagent for 10 minutes and stained with LIVE/DEAD Aqua stain, CD3-APC-H7 (BD Biosciences; Cat# 641406), CD4-BV785 (BioLegend; Cat# 317442), CD8-PacBlue (Invitrogen; Cat# MHCD0828), CD14-BV650 (BioLegend; Cat# 301836), CD16-PerCP/Cy5.5 (BioLegend; Cat# 302028), CD19-BV605 (BD Biosciences; Cat# 562653), CD20-BV570 (BioLegend; Cat# 302332), CD27-Alx700 (BioLegend; Cat# 302814), CD32-PE (BioLegend; Cat# 303206); CD45RO-ECD (Beckman Coulter; Cat# IM2712U), CD123-PE/Cy5 (BD Biosciences; Cat# 551065), and TCRγδ-APC (BD Biosciences; Cat# 555718). CD4+ T cells were isolated by fluorescence-activated cell sorting (FACS) on a FACSAria (Becton Dickinson) using previously described protocols32 (link).
Sun C., Liu L., Pérez L., Li X., Liu Y., Xu P., Boritz E.A., Mullins J.I, & Abate A.R. (2022). Droplet microfluidic sequencing of HIV proviruses with their integration sites in patients receiving antiretroviral therapy. Nature biomedical engineering, 6(8), 1004-1012.
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2

Isolation and Characterization of CD4+ T Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Participant recruitment and informed consent were performed under Institutional Review Board (IRB)-approved protocols at the US National Institutes of Health (NIH) and University of Washington. Peripheral blood mononuclear cells (PBMC) were isolated from whole blood by density gradient centrifugation. PBMC were incubated with Fcγ-receptor-blocking reagent for 10 minutes and stained with LIVE/DEAD Aqua stain, CD3-APC-H7 (BD Biosciences; Cat# 641406), CD4-BV785 (BioLegend; Cat# 317442), CD8-PacBlue (Invitrogen; Cat# MHCD0828), CD14-BV650 (BioLegend; Cat# 301836), CD16-PerCP/Cy5.5 (BioLegend; Cat# 302028), CD19-BV605 (BD Biosciences; Cat# 562653), CD20-BV570 (BioLegend; Cat# 302332), CD27-Alx700 (BioLegend; Cat# 302814), CD32-PE (BioLegend; Cat# 303206); CD45RO-ECD (Beckman Coulter; Cat# IM2712U), CD123-PE/Cy5 (BD Biosciences; Cat# 551065), and TCRγδ-APC (BD Biosciences; Cat# 555718). CD4+ T cells were isolated by fluorescence-activated cell sorting (FACS) on a FACSAria (Becton Dickinson) using previously described protocols32 (link).
Sun C., Liu L., Pérez L., Li X., Liu Y., Xu P., Boritz E.A., Mullins J.I, & Abate A.R. (2022). Droplet microfluidic sequencing of HIV proviruses with their integration sites in patients receiving antiretroviral therapy. Nature biomedical engineering, 6(8), 1004-1012.
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