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Cell culture components

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Cell culture components are a range of laboratory equipment and supplies used to support the growth and maintenance of cells in a controlled, sterile environment. These components include items such as culture media, plates, flasks, and other essential tools required for cellular propagation and experimentation.

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Lab products found in correlation

Etoposide, by Enzo Life Sciences (2 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Recombinant human tgf β1, by R&D Systems (1 mentions) Recombinant human igf2, by R&D Systems (1 mentions)

2 protocols using cell culture components

1

Quantifying IGF-I and IGFBP-4 in Cell Culture

Cited 1 time
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Cell culture components were from Gibco Life Technologies (Grand Island, NY). Bovine serum albumin (BSA) and Sm1.2 anti-IGF-I antibody were from Sigma Millipore (Temecula, CA). Recombinant human TGF-β1 and Quantikine IGF-I ELISA kits were from R&D Systems (Minneapolis, MN). Etoposide was obtained from Enzo Life Sciences (Farmingdale, NY). Total IGFBP-4 ELISA kits and monoclonal antibody generated against a unique exosite in PAPP-A (mAb-1/41) (Mikkelsen et al., 2008 (link); Mikkelsen et al., 2014 (link)) were obtained from Ansh Labs (Webster, TX).
Bale L.K., Schafer M.J., Atkinson E.J., LeBrasseur N.K., Haak A.J., Oxvig C, & Conover C.A. (2022). Pregnancy-associated Plasma Protein-A (PAPP-A) is a Key Component of an Interactive Cellular Mechanism Promoting Pulmonary Fibrosis. Journal of cellular physiology, 237(4), 2220-2229.
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2

Quantification of IGFBP-4 and PAPP-A

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell culture components were from Gibco Life Technologies. Bovine serum albumin (BSA) was from MilliporeSigma. Recombinant human IGF-II was from R&D Systems and IGFBP-4 was a gift from our colleague, Professor Claus Oxvig (Aarhus University, Denmark). Anti-IGFBP-4 antibodies were purchased from Abcam: N-terminal ab92625 and C-terminal ab77350. Secondary antibodies were from LiCor, Inc: Donkey anti-goat (926–68074) and donkey anti-rabbit (926–32213). Etoposide was obtained from Enzo Life Sciences. picoPAPP-A ELISA kits and monoclonal antibody generated against a unique exosite in PAPP-A (mAb-PA1/41)38 (link), 39 (link) were obtained from Ansh Labs (Webster, TX).
Conover C.A, & Bale L.K. (2022). Senescence Induces Proteolytically-active PAPP-A Secretion and Association with Extracellular Vesicles in Human Pre-adipocytes. Experimental gerontology, 172, 112070.
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