Tau fibrils or tau fibrils-chaperone mixtures (10 µl) were deposited on glow-discharged carbon-coated copper EM grids (Electron Microscopy Sciences), washed with three consecutive drops of 1% w/v Uranyl-formate, and air-dried. Imaging was performed on an FEI T12 Spirit transmission electron microscope at 120 kV and a magnification of 9300–30,000 times, equipped with a Gatan OneView CMOS 4K × 4K CCD camera.
Glow discharged carbon coated copper em grids
Manufactured by Electron Microscopy Sciences
Glow-discharged carbon-coated copper EM grids are specimen support films used in transmission electron microscopy (TEM) applications. They feature a carbon coating that is exposed to a glow discharge treatment, which modifies the surface properties to enhance specimen adherence and contrast.
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Lab products found in correlation
2 protocols using glow discharged carbon coated copper em grids
1
Tau Fibrils Imaging by TEM
2
Tau Fibrils Electron Microscopy
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Tau fibrils or tau fibrils-chaperone mixtures (10 µl) were deposited on glow discharged carbon-coated copper EM grids (Electron Microscopy Sciences), washed with three consecutive drops of 1% w/v Uranylformate, and air-dried. Imaging was performed on an FEI T12 Spirit transmission electron microscope at 120kV and a magnification of 9300-30000 times, equipped with a Gatan OneView CMOS 4K x 4K CCD camera.
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