Quercetin and chloroquine were obtained from Sigma-Aldrich; Primary antibodies for western boltting are as follows: TFEB (Cell Signaling Technology, Danvers, MA, USA), a-tubulin (Sigma-Aldrich, St. Louis, MO, USA), LAMP-1 (Santa Cruz, Santa Cruz, CA, USA), FTL (Abcam, USA), LaminA/C (Santa Cruz, Santa Cruz, CA, USA), GAPDH (Santa Cruz); TFEB siRNA was purchased from Life Technologies. (Santa Cruz), FTL (Abcam), LaminA/C (Santa Cruz), GAPDH (Santa Cruz, Santa Cruz, CA, USA) were used in this study. TFEB siRNA was purchased from Life Technologies; Fe on assay kit, MDA assay kit, and carbonylated protein ELISA kit were purchased from Nanjing Jiancheng Biological Engineering (Nanjing, China). The cytoplasmic cytosolic protein extraction and isolation kits were purchased from Beyotime (Beijing, China). Breast cancer cell lines were sourced from the National Collection of Authenticated Cell Cultures.
An S, & Hu M. (2022). Quercetin Promotes TFEB Nuclear Translocation and Activates Lysosomal Degradation of Ferritin to Induce Ferroptosis in Breast Cancer Cells. Computational Intelligence and Neuroscience, 2022, 5299218.
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