Geranylgeranyl pyrophosphate (GGPP), cycloheximide, trypsin, and digitonin were purchased from Sigma-Aldrich. digitonin was washed and recrystallized in ethanol three times according to the manufacturer's instructions. Lovastatin was a gift from Merck & Co (Rahway NJ). GGSPP and 2-fluoro-GGPP were gifts from Reuben Peters (Iowa State University) and Philip Zerbe (University of California Davis). Fos-Choline-13 and Decyl Maltose Neopentyl Glycol were purchased from Anatrace. Anti-myc 9E10 supernatant was prepared from cells (CRL 1729, American Type Culture Collection) cultured in RPMI1640 medium (GIBCO BRL) with 10% fetal calf serum. Living colors mouse anti-GFP monoclonal antibody was purchased from Clontech, and HRP-conjugated goat antimouse antibody was purchased from Jackson ImmunoResearch.
Living colors mouse anti gfp monoclonal antibody
Manufactured by Takara Bio
The Living Colors Mouse Anti-GFP Monoclonal Antibody is a primary antibody that specifically binds to the green fluorescent protein (GFP). It is designed for use in various immunodetection applications to detect and visualize GFP-tagged proteins.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Trypsin, by Merck Group (2 mentions)
Cycloheximide (chx), by Merck Group (2 mentions)
Lovastatin, by Merck Group (2 mentions)
Digitonin, by Merck Group (2 mentions)
Crl1729, by American Type Culture Collection (2 mentions)
Decyl maltose neopentyl glycol, by Anatrace (2 mentions)
Geranylgeranyl pyrophosphate ggpp, by Merck Group (2 mentions)
Fos choline 13, by Anatrace (2 mentions)
Monoclonal rabbit anti ha antibody, by Abcam (2 mentions)
Pstaire cdc28 antibody, by Thermo Fisher Scientific (2 mentions)
Hrp conjugated goat anti mouse antibody, by Jackson ImmunoResearch (1 mentions)
4 protocols using living colors mouse anti gfp monoclonal antibody
1
Purification and Analysis of Prenylated Proteins
2
Preparation and Characterization of Geranylgeranyl Pyrophosphate
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Geranylgeranyl pyrophosphate (GGPP), cycloheximide, trypsin, and digitonin were purchased from Sigma-Aldrich. digitonin was washed and recrystallized in ethanol 3 times according to the manufacturer's instructions. Lovastatin was a gift from Merck & Co (Rahway NJ). GGSPP and 2-fluoro-GGPP were gifts from Reuben Peters (Iowa State University) and Philip Zerbe (University of California Davis). Fos-Choline-13 and Decyl Maltose Neopentyl Glycol were purchased from Anatrace. Anti-myc 9E10 supernatant was prepared from cells (CRL 1729, American Type Culture Collection) cultured in RPMI1640 medium (GIBCO BRL) with 10% fetal calf serum. Living colors mouse anti-GFP monoclonal antibody was purchased from Clontech,and goat anti-mouse antibody HRP-conjugated antibody was purchased from Jackson ImmunoResearch.
3
Extracting and Detecting Proteins
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Protein extraction was modified from (Meimoun et al., 2000 ). After precipitation with TCA as described, samples were washed once with acetone and boiled 5 min with equivolume urea buffer (8M urea, 4M 2‐mercaptoethanol, 125 mM Tris pH 6.8 and 10% SDS), then centrifuged 5 min at 13 krpm. The supernatant was mixed with equivolume 20% glycerol + bromophenol blue and run on SDS‐PAGE gels for Western blotting. The HA tag was detected with monoclonal rabbit anti‐HA antibody from Abcam. GFP was detected with Living Colors monoclonal mouse anti‐GFP antibody from Clontech. PSTAIRE (Cdc28) antibody was obtained from Invitrogen. Secondary IgG antibodies (anti‐mouse or anti‐rabbit) were obtained from Bio‐Rad.
4
Protein Extraction and Western Blotting
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