The procedure for IVT injection has been described previously [29 (link),75 (link),76 (link)]. Briefly, prior to injection animals were anesthetized with 150 mg/kg body weight (BW) ketamine hydrochloride (Parnell Laboratories, New South Wales, Australia) and 10 mg/kg BW xylazine hydrochloride (Troy Laboratories, New South Wales, Australia) and their pupils were dilated with a topical application of 1% tropicamide and 2.5% phenylephrine hydrochloride (Alcon Laboratories Inc., Fort Worth, TX, USA). For the injection, a 1 µL aliquot of solution containing each chemical was injected into the vitreous body of both eyes of each mouse using a Nanofil microsyringe with a 33G needle (World Precision Instruments Inc., Sarasota, FL, USA), under an ocular surgery microscope (Leica Microsystems GmbH, Wetzlar, Germany). A bilateral approach was taken to minimize the number of animals used in this study. All procedures were performed carefully to avoid injuring the lens or retina. Four or seven days following injections, the eyes were collected and subjected to further analysis as described below. Eyes with bleeding or inflammation caused by technical errors were excluded from analysis.
33 g needle
Manufactured by World Precision Instruments
Sourced in United States
The 33 G needle is a precision laboratory instrument designed for delicate and precise fluid handling tasks. It features a thin 33 gauge needle and is constructed with high-quality materials to ensure reliable performance. The core function of this product is to provide a precise and controlled method for transferring small volumes of liquids or gases in a laboratory setting.
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Lab products found in correlation
3 protocols using 33 g needle
1
Intravitreal Injection Procedure in Mice
2
Purified Ly6G+ Cells Sciatic Nerve Injection
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Purified Ly6G+ cells were resuspended in sterile PBS at a concentration of 1 × 105 cells/μl and loaded into a Nanofil syringe with a 33 G needle (World Precision Instrument). Each sciatic nerve was injected with 1.5 μl of cell suspension.
3
Purified Ly6G+ Cells Sciatic Nerve Injection
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