Flow cytometric analysis of immune inflammatory cells was performed using cell type-specific monoclonal antibodies on 3-day e-cig aerosol-exposed samples. Briefly, 2.0–4.0 × 105 BAL cells were stained with cell type-specific markers in 1x PBS for 30 min, followed by washing and re-suspension in 0.1 mL of 1x PBS for analysis. Cell-specific markers such as LY6B.2 Alexa fluor488-conjugated antibody for neutrophils (Novus Biologicals Cat# NBP213077AF488), CD8a PE-cy5-conjugated antibody for T-lymphocytes (BD Biosciences 553034), and F4/80 PE-conjugated antibody for macrophages (BioLegend Cat #123109) were used. Total macrophage, neutrophil and CD4a+ and CD8a+ lymphocyte counts in BALF were determined by taking the percentage of each cell type observed and multiplying that by the total cell counts. Flow cytometry data acquisition was performed using a Guava® easyCyte™ flow cytometer (Millipore Sigma) and analyzed using Guava® InCyte™ software.
F4 80 pe conjugated antibody
Manufactured by BioLegend
The F4/80 PE-conjugated antibody is a cell surface marker that is commonly used to identify and study macrophages in various biological systems. It is a fluorescently labeled monoclonal antibody that specifically binds to the F4/80 antigen, which is expressed on the surface of mature mouse macrophages. The PE (Phycoerythrin) fluorescent dye allows for the detection and quantification of F4/80-positive cells using flow cytometry or other fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using f4 80 pe conjugated antibody
1
E-Cigarette Aerosol Immunophenotyping
2
Quantifying Inflammatory Cell Types
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Flow cytometric analysis of immune inflammatory cells was performed using cell type‐specific monoclonal antibodies on 3‐day e‐cig aerosol‐exposed samples. Briefly, 2.0‐4.0 × 105 BAL cells were stained with cell type‐specific markers in 1× PBS for 30 minutes, followed by washing and re‐suspension in 0.1 mL of 1x PBS for analysis. Cell‐specific markers such as LY6B.2 Alexa fluor488‐conjugated antibody for neutrophils (Novus Biologicals Cat# NBP213077AF488), CD8a PE‐cy5‐conjugated antibody for T lymphocytes (BD Biosciences 553034), and F4/80 PE‐conjugated antibody for macrophages (BioLegend Cat #123109) were used. Total macrophage, neutrophil and CD4a+ and CD8a+ lymphocyte counts in BALF were determined by taking the percentage of each cell type observed and multiplying that by the total cell counts. Flow cytometry data acquisition was performed using a Guava® easyCyte™ flow cytometer (Millipore Sigma) and analyzed using Guava® InCyte™ software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!