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Deoxynojirimycin

Manufactured by Cayman Chemical
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Deoxynojirimycin is a synthetic compound that functions as an alpha-glucosidase inhibitor. It is commonly used in biochemical research applications.

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Lab products found in correlation

1 3 dithiothreitol, by Roche (1 mentions) Mg132, by Merck Group (1 mentions) Doxycycline, by Merck Group (1 mentions) Anisomycin, by Merck Group (1 mentions) Bafilomycin a1, by LC Laboratories (1 mentions) Bafilomycin a1 b 1080, by LC Laboratories (1 mentions)

2 protocols using deoxynojirimycin

1

Comprehensive Protein Manipulation Protocol

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The following reagents were used at working concentrations as listed: 50 μg/ml cycloheximide (C-1189; AG Scientific), 10 μM MG-132 (474791; Sigma-Aldrich), 10 μM NMS-873 (6180/25; R&D), 0.5 μM anisomycin (A9789; Sigma-Aldrich), 2.5 mM deoxynojirimycin (Cayman Chemicals), 0.1 μM thapsigargin (586005; EMD), 0.5 mM 1,3-dithiothreitol (10708984001; Roche), 250 nM bafilomycin A1 (B-1080; LC Laboratories), 0.5 μg/ml doxycycline (D9891; Sigma-Aldrich), 2 μg/ml puromycyin (P9620; Sigma-Aldrich), and 300 mg/ml G418 sulfate (G-1033; AG Scientific).
Cheatham A.M., Sharma N.R, & Satpute-Krishnan P. (2023). Competition for calnexin binding regulates secretion and turnover of misfolded GPI-anchored proteins. The Journal of Cell Biology, 222(10), e202108160.
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2

Simultaneous Lysosome and PrP* Imaging

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YFP-PrP* NRK or parental NRK cells were transfected with Lamp1-mCherry using Lipofectamine 3000. 24 h after transfection, cells were seeded onto #1.5 glass coverslips. 48 h after transfection, cells were incubated with 0.4 μg/ml Alexa Fluor 647-conjugated anti-GFP antibody (A-31852; Thermo Fisher Scientific) and 250 nM Bafilomycin A1 (B-1080; LC Laboratories) for time-lapse imaging. Where indicated, 2.5 mM deoxynojirimycin (Cayman Chemicals) was included in the medium.
Cheatham A.M., Sharma N.R, & Satpute-Krishnan P. (2023). Competition for calnexin binding regulates secretion and turnover of misfolded GPI-anchored proteins. The Journal of Cell Biology, 222(10), e202108160.
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