Blood samples were collected from patients in fasting states. From each patient, 5–8 mL of whole blood was drawn into vacutainers and centrifuged at 1000× g for 20 min at 25 °C to separate blood cells and serum. After collection, serum samples were aliquoted and stored at −80 °C until further analysis. Serum iron and transferrin (Cobas Integra analyser from Roche, Basel, Switzerland), and serum ferritin (AxSym analyzer from Abbott, Chicago, IL, USA) were immediately determined. A colorimetric (Ab932715, AbCam, Cambridge, UK) and an enzyme-linked immunosorbent assay (Ab288174, AbCam, Cambridge, UK) were used to measure total iron-binding capacity (TIBC) following manufacturer instruction. Briefly, Human Anti-Transferrin antibodies were used to measure the amount of transferrin in the sample. In parallel, iron standard solutions were added to serum samples to saturate the transferrin. The amount of iron remaining free was then measured by colorimetry at a neutral pH using deferoxamine mesylate as iron chelator. Total iron was then measured after acidification by HCl. All measurements were used to calculate total iron binding capacity of the biological samples.
Cobas integra analyser
Manufactured by Roche
Sourced in Switzerland
The Cobas Integra analyzer is a fully automated, random-access clinical chemistry and immunochemistry analyzer designed for in-vitro diagnostic testing. The Cobas Integra is capable of performing a wide range of clinical chemistry and immunoassay tests on a variety of sample types, including serum, plasma, and urine.
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Lab products found in correlation
2 protocols using cobas integra analyser
1
Measurement of Serum Iron Biomarkers
2
Comprehensive Blood Analysis Protocol
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Blood samples were collected before exercise (i.e., after 5 min of seated rest) from the right forearm vein and 5 min after exercise from the left forearm vein to avoid local inflammation caused by the first sample. Three tubes were used for blood collection. The first with heparin anticoagulant contained 5 ml of blood to measure the selected parameters, i.e. urea (URE), aspartate aminotransferase (AST), creatinine (CRE), creatine kinase (CK), uric acid (UA) and glutathione peroxidase (GPx). The second, 3 ml of blood in a tube with ethylenediaminetetraacetic acid (EDTA), was used to determine the total blood count. The total blood count values are reported as numbers, i.e., white blood cells (WBC), monocytes (MO), lymphocytes (LY), granulocytes (GR), red blood cells (RBC) and haemoglobin (HB). The third contained 3 ml of blood in a fluorinated tube to measure plasma lactate [La] and glucose (GLC). In order to eliminate inter-assay variance, all samples were analysed in the same assay run. All assays were performed in duplicate in the same laboratory with simultaneous use of a control serum from Randox on a Cobas Integra analyser (Roche Diagnostics, Switzerland). All methods used in the analysis of the current study are presented in Table 1 .
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