All experimental mice were male and female adult mice on a C57BL/6J background strain. Wild-type C57BL/6J mice were purchased as adults from Jackson Laboratory, and all transgenic lines were bred in our animal facility. CRF-ires-Cre (CRF-Cre)18 (link),39 (link) and VGLUT2-ires-Cre (VGLUT2-Cre)40 (link) mice were bred with WT C57BL/6J mice, and hemizygous CRF-Cre mice were bred with homozygous floxed Ai9-tdTomato or floxed EGFP-L10a mice purchased from Jackson Laboratory (stocks 007909 and 024750) to produce CRF-Cre-reporter mice. Mice were group-housed with ad libitum access to food and water in colony room on a 12:12 h reverse light cycle, with lights off at 7:30 a.m. Mice were singly housed for one week prior to the onset of behavioral experiments and remained singly housed thereafter. Experiments began approximately 3 h into the dark phase of the light cycle. All experimental procedures were approved by the Institutional Animal Care and Use Committees at Weill Cornell Medicine and the University of North Carolina-Chapel Hill.
Floxed egfp l10a
Manufactured by Jackson ImmunoResearch
The Floxed EGFP-L10a is a genetically modified mouse line that expresses enhanced green fluorescent protein (EGFP) tagged with the large ribosomal protein L10a. The EGFP-L10a fusion protein is flanked by loxP sites, allowing for conditional expression or deletion of the reporter in a cell-type specific manner.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using floxed egfp l10a
1
Transgenic Mouse Models for CRF and VGLUT2 Studies
2
Genetic Mouse Models for Stress Research
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All experimental mice were male and female adult mice on a C57BL/6J background strain. Wild-type C57BL/6J mice were purchased as adults from Jackson Laboratory, and all transgenic lines were bred in our animal facility. CRF-ires-Cre (CRF-Cre) 15, (link)36 (link) and VGLUT2-ires-Cre (VGLUT2-Cre) 37 (link) mice were bred with WT C57BL/6J mice, and hemizygous CRF-Cre mice were bred with homozygous floxed Ai9-tdTomato or floxed EGFP-L10a mice purchased from Jackson Laboratory (stocks 007909 and 024750) to produce CRF-Cre-reporter mice. Mice were group housed with ad libitum access to food and water in colony room on a 12:12 hr reverse light cycle, with lights off at 7:30 a.m. Mice were singly housed for one week prior to the onset of behavioral experiments and remained singly housed thereafter. Experiments began approximately 3 hr into the dark phase of the light cycle. All experimental procedures were approved by the Institutional Animal Care and Use Committees at Weill Cornell Medicine and University of North Carolina-Chapel Hill.
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