Anti h2a

Manufactured by Active Motif

Anti-H2A is a laboratory reagent that specifically binds to the histone protein H2A. It is used in various applications in epigenetic research and chromatin studies.

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Anti-H2A.Z (3 citations)

2 protocols using anti h2a

Chromatin Immunoprecipitation Profiling Antibodies

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The following commercially available antibodies were used:
Anti-H3 (1:5.000, #61475), Anti-H3K36me3 (1:4.000, #61102), Anti-H3K36me2 (1:1.000, #39255), Anti-H3K36me1 (1:1.000, #61351), Anti-H3K4me3 (1:500, #61379) and anti-H2A (1:1.000, #39209) were from Active Motif. Anti-H3K27me3 (1:1.000, C15410069), Anti-H3K79me3 (1:1.000, C15310068), anti-H2A.Zac (2 µg/IP, C15410202-050) and Anti-H3K9me3 (1:1.000, C15410056) were from Diagenode. Anti-H4K20me3 (1:1.000, ab9053), Anti-H3 (1:30.000, ab1791), anti-MTA1 (1:1.000, ab71153 or 3 µg/IP, ab50209), anti-CHD4 (1:1.000 or 2 µg/IP, ab70469), anti-HDAC2 (1:5.000, ab124974) anti-RBBP4 (1:1.000, ab488), anti-RBBP7 (1:1.000, ab3535), Anti-H3K27ac (2 µg/IP, ab4729), anti-H2A.Z (1:3.000, ab4174), and anti-MBD3 (1:5.000, ab157464) were from Abcam. Anti-PWWP2A (1:1.000, NBP2-13833) from Novus (Acris). Anti-HA-HRP (1:40.000, 2999S) and anti-HDAC1 (1:20.000, 5356S) from Cell Signaling Technology. Anti-FLAG M2 (1:80.000 or 3 µg/IP, F1804) and anti-mouse IgG (3 µg/IP, I8765) from Sigma-Aldrich.
The following secondary antibodies were used:
Anti-rabbit IRDye 800CW (1:10.000, 926-32211) and anti-mouse IRDye 680RD (1:10.000 926‐68070) from LI-COR Biosciences. Anti-mouse-HRP (1:10.000, M114) from Leinco Technologies. Anti-mouse and anti-rabbit HRP-linked antibodies (1:10.000, NA931 and NA934) from VWR.

Link S., Spitzer R.M., Sana M., Torrado M., Völker-Albert M.C., Keilhauer E.C., Burgold T., Pünzeler S., Low J.K., Lindström I., Nist A., Regnard C., Stiewe T., Hendrich B., Imhof A., Mann M., Mackay J.P., Bartkuhn M, & Hake S.B. (2018). PWWP2A binds distinct chromatin moieties and interacts with an MTA1-specific core NuRD complex. Nature Communications, 9, 4300.

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Antibodies for Immunoblot Detection

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Antibodies for immunoblots were anti-Rad53 (Abcam, ab 104232) dilution: 1:2000, anti-Orc6 (SB49, a kind gift from Steve Bell) dilution: 1: 3000, anti-Mcm7 (Santa Cruz, yN-19, Sc 6688) dilution: 1:2000, anti-FLAG M2 peroxidase (Sigma, F 3165) (for Yta7/Mutants-FLAG_3x) dilution 1: 5000, anti-TAP (Thermo Fisher, CAB1001) dilution 1: 5000, anti-acetyl histone H3 (Millipore 06-599) dilution 1: 2000, anti-H2A (Active Motif, 39235) dilution 1: 2000 and anti-H3 (Abcam, ab 1791) dilution 1: 5000.

Chacin E., Bansal P., Reusswig K.U., Diaz-Santin L.M., Ortega P., Vizjak P., Gómez-González B., Müller-Planitz F., Aguilera A., Pfander B., Cheung A.C, & Kurat C.F. (2021). A CDK-regulated chromatin segregase promoting chromosome replication. Nature Communications, 12, 5224.

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