Mice were sacrificed by carbon dioxide asphyxiation or deep anesthesia with isoflurane followed by cervical dislocation. Mouse brains were manually dissected under a fluorescent stereoscope (Zeiss, Olympus or Leica). Brightfield and/or GFP fluorescent images were taken for the dissected brain, and overlaid using ImageJ58 (link). Brains were then fixed in 4% formaldehyde or 10% formalin for 48 to 96 hours, embedded in paraffin, sectioned at 6 μm and stained with hematoxylin and eosin (H&E) for pathology. For tumor size quantification, H&E slides were scanned using an Aperio digital slidescanner (Leica). Tumors were manually outlined as region-of-interest (ROI), and subsequently quantified using ImageScope (Leica). Sections were de-waxed, rehydrated and stained using standard immunohistochemistry (IHC) protocols as previously55 (link), 59 (link). The following commonly used antibodies were used for IHC: rabbit anti-Ki67 (abcam ab16667, 1:500), rabbit anti-GFP (ThermoFisher Scientific A11122, 1:300) rabbit anti-GFAP (Dako, 1:500), and mouse anti-Cas9 (Diagenode, 1:300).
A11122
Manufactured by Agilent Technologies
The A11122 is a high-precision digital multimeter designed for laboratory and industrial applications. It features a wide range of measurement capabilities, including voltage, current, resistance, and capacitance. The device offers accurate and reliable performance, with a user-friendly interface and intuitive controls.
Automatically generated - may contain errors
2 protocols using a11122
1
Detailed Brain Dissection and Histological Analysis
2
Detailed Brain Dissection and Histological Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mice were sacrificed by carbon dioxide asphyxiation or deep anesthesia with isoflurane followed by cervical dislocation. Mouse brains were manually dissected under a fluorescent stereoscope (Zeiss, Olympus or Leica). Brightfield and/or GFP fluorescent images were taken for the dissected brain, and overlaid using ImageJ58 (link). Brains were then fixed in 4% formaldehyde or 10% formalin for 48 to 96 hours, embedded in paraffin, sectioned at 6 μm and stained with hematoxylin and eosin (H&E) for pathology. For tumor size quantification, H&E slides were scanned using an Aperio digital slidescanner (Leica). Tumors were manually outlined as region-of-interest (ROI), and subsequently quantified using ImageScope (Leica). Sections were de-waxed, rehydrated and stained using standard immunohistochemistry (IHC) protocols as previously55 (link), 59 (link). The following commonly used antibodies were used for IHC: rabbit anti-Ki67 (abcam ab16667, 1:500), rabbit anti-GFP (ThermoFisher Scientific A11122, 1:300) rabbit anti-GFAP (Dako, 1:500), and mouse anti-Cas9 (Diagenode, 1:300).
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