HA sodium salt (molecular weight = 10–12 kDa) was purchased from Lifecore Biomedical (MN, USA). Methoxy poly(ethylene glycol) amine (PEG‐NH2, molecular weight = 5 kDa) was obtained from Laysan Bio Inc. (AL, USA). Cy5.5‐DBCO and Ac4ManNAz were purchased from Click Chemistry Tools (AZ, USA). Thiazolyl blue tetrazolium bromide (MTT), DBCO‐amine and dimethylformamide (DMF) were obtained from Sigma‐Aldrich Co. (MO, USA). Sodium cyanoborohydride (NaBH3CN) was purchased from TCI (Tokyo, Japan). Poly(vinylidene fluoride) membrane was obtained from Millipore (MA, USA). Cy5.5 NHS ester dye was purchased from GE Healthcare (IL, USA). Foetal bovine serum (FBS), RPMI 1640, Dulbecco's phosphate‐buffered saline (DPBS), antibiotic‐antimycotic (AA) solution (100×) and trypsin‐trypsin‐ethylene diamine tetraacetic acid were purchased from WelGENE (Gyeongsan, Korea). NIH/3T3, CT26, RAW264.7, MDA‐MB‐231 and HCT‐116 were provided by the Korean Cell Line Bank (Seoul, Korea). Water was purified using an AquaMax‐Ultra water purification system from Younglin Co. (Anyang, Korea). All other chemicals were of analytical grade and used without further purification. Information on the antibodies used in the experiments is listed in Table S1 .
Ha sodium salt
Manufactured by Lifecore Biomedical
Sourced in United States
HA sodium salt is a high-purity hyaluronic acid (HA) product. HA is a naturally occurring polysaccharide found in the human body. The sodium salt form of HA is a key component in various biomedical and pharmaceutical applications.
Automatically generated - may contain errors
Lab products found in correlation
Polyvinylidene fluoride membrane, by Merck Group (1 mentions)
Cy5.5 nhs ester dye, by GE Healthcare (1 mentions)
Hct116, by Korean Cell Line Bank (1 mentions)
Mda mb 231, by Korean Cell Line Bank (1 mentions)
Ac4mannaz, by Vector Laboratories (1 mentions)
Cy5.5 dbco, by Vector Laboratories (1 mentions)
Dulbecco s phosphate buffered saline dpbs, by Welgene (1 mentions)
Nih3t3, by Korean Cell Line Bank (1 mentions)
Raw 264, by Korean Cell Line Bank (1 mentions)
Dimethylformamide dmf, by Merck Group (1 mentions)
Aquamax ultra water purification system, by Younglin (1 mentions)
Sodium ascorbate, by Merck Group (1 mentions)
Copper 2 sulfate pentahydrate, by Thermo Fisher Scientific (1 mentions)
Tris 3 hydroxypropyltriazolylmethyl amine, by Merck Group (1 mentions)
N hydroxysuccinimide, by Thermo Fisher Scientific (1 mentions)
2 n morpholino ethanesulfonic acid sodium salt, by Merck Group (1 mentions)
N hydroxysuccinimide, by Tokyo Chemical Industry (1 mentions)
3 protocols using ha sodium salt
1
Glycan-Mediated Tumor Cell Targeting
2
Synthesis and Characterization of Click-Functionalized Peptides
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We purchased HA sodium salt (molecular weight 16 kDa) from Lifecore Biomedical; 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide from TCI Chemicals; 2-(N-morpholino)ethane-sulfonic acid sodium salt, tris(3-hydroxypropyltriazolylmethyl)amine, and sodium ascorbate from Sigma-Aldrich; and 11-azido-3,6,9-trioxaundecan-1-amine, N-hydroxysuccinimide, and copper (II) sulfate pentahydrate from Acros Organics. Alkyne-functionalized peptides bearing an N-terminal 4-pentynoic acid (homopropargyl) modification, hpP79 and aoP79, were obtained from Biomatik. Additionally, alkyne-functionalized peptides bearing an N-terminal alkyne polyethylene glycol group modification, 2.5HIP and a control p79 with two permuted amino acids inactive on BDC2.5 T cells, were purchased from PolyPeptide. All reagents were used as received without further purification.
3
Synthesis of Hyaluronic Acid Hydrogel
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All chemicals were purchased from Sigma-Aldrich and cell culture reagents from ThermoFisher Scientific unless stated otherwise. HA-SH synthesis: 400 mg (1 mmol of the disaccharide repeat unit) of HA sodium salt (Lifecore Biomedical, 1.01-1.8 MDa), and 23.8 mg (0.1 mmol) of 3,3'-dithiobis(propanoic dihydrazide) (DTPHY, Frontier Scientific) were dissolved in a 150 mM MES solution with occasional gentle swirling (final pH 4.1). Then 38.4 mg (0.2 mmol) of 1-ethyl-3-(3dimethylaminopropyl)carbodiimide (EDC, Fluka) was dissolved in 1 ml of deionized water and added dropwise with stirring. The stirring was stopped and the reaction was allowed to continue overnight. The pH increased towards ~4.5 during the course of the reaction, with MES buffer preventing it from going higher. Then, 143.33 mg (0.5 mmol) of TCEP-HCl (Fluorochem) was dissolved in 500 µl water and added, the solution was homogenized by swirling, and the reduction was left to proceed overnight in a standing sealed flask. Finally, 1 g (17 mmol) of NaCl was added to the solution, and the mixture was dialyzed against ultrapure water balanced to pH 4.5 with dilute HCl, with 4 water changes over 24h, to yield a solution of pure HA-SH sodium salt.
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