Pcsii ef mcs

Manufactured by Addgene

The PCSII-EF-MCS is a plasmid vector designed for gene expression in mammalian cells. It contains an enhanced CMV promoter and multiple cloning site for the insertion of genes of interest. The vector also includes a neomycin resistance gene for selection of stable transfectants.

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Lab products found in correlation

L7 55, by Beckman Coulter (1 mentions) Hek293tn cells, by System Biosciences (1 mentions) Pspax2, by Addgene (1 mentions) Pmd2 g, by Addgene (1 mentions)

1 protocol using pcsii ef mcs

Lentiviral Vectors for Cell Cycle Monitoring

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lentiviral vectors mCherry-hCdt1(30/120)/pCSII-EF-MCS (DDBJ/EMBL/GenBank, AB512478) and mVenus-hGeminin(1/100)/pCSII-EF-MCS (DDBJ/EMBL/GenBank, AB512479) were purchased from the Riken Brain Science Institute, Japan (Dr. Atsushi Miyawaki, head of provider laboratory, and Dr. Hiroyuki Miyoshi, developer of pCSII-EF-MCS). Lentiviral particles were generated by co-transfection of HEK-293TN cells (System Biosciences) with mCherry-hCdt1 (30/120)/pCSII-EF-MCS or mVenus-hGeminin (1/100)/pCSII-EF-MCS lentiviral vectors, alongside the packaging plasmid psPAX2 (Addgene plasmid, #12260) and the envelope plasmid pMD2.G (Addgene plasmid, #12259). The culture supernatant was collected and concentrated by ultracentrifugation at 22,000 rpm for 3 h (Beckman L7-55 with SW32Ti rotor) at 4 °C. The virus titer was estimated by transduction on HeLa cells and subsequent flow cytometric analysis for fluorescent protein expression.

Taïeb H.M., Garske D.S., Contzen J., Gossen M., Bertinetti L., Robinson T, & Cipitria A. (2021). Osmotic pressure modulates single cell cycle dynamics inducing reversible growth arrest and reactivation of human metastatic cells. Scientific Reports, 11, 13455.

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