Milli q millipore system advantage a10

Human skin fibroblasts (CRL-1474) were obtained from the American Type Culture Collection (ATCC). Sterile reagents for cell culture, including AlgiMatrix 3D Cell Culture System, were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Phospholipid and ceramide internal standards were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). All chemicals were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). All solvents were of LC-MS grade. Milli-Q water was used for all experiments, filtered through a 0.22 µm filter and obtained using a Milli-Q Millipore system (Advantage A10, Millipore Corporation, Billerica, MA, USA).

Internal standards for phospholipids and ceramides were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). All chemicals were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA); all solvents were of LC–MS grade. Milli-Q water was used for all experiments, filtered through a 0.22 μm filter, and obtained using a Milli-Q Millipore system (Advantage A10, Millipore Corporation, Billerica, MA, USA).