Rabbit anti sdf 1 cxcl12 polyclonal antibody

The expression of SDF-1 and its presence and location were evaluated by IF staining using rabbit anti-SDF-1/CXCL12 polyclonal antibody (Bioss, Woburn, MA, USA). Deparaffinized and rehydrated sections were blocked with 5% bovine serum albumin for 60 min at room temperature (25 °C). Sections were incubated with primary antibody (rabbit anti-SDF-1/CXCL12 polyclonal antibody; Bioss) (1:100 dilution) overnight at 4 °C. Secondary antibodies (Donkey Anti-Rabbit IgG H&L Alexa Fluor® 594; Abcam, Cambridge, UK) (1:200 dilution) were used to incubate the sections for 40 min in room temperature. Quenching of autofluorescence (Vector TrueVIEW; Vector Labs, Burlingame, CA, USA) was performed to remove unwanted fluorescence in the tissue sections following the manufacturer’s protocol. Sections were mounted with VECTASHIELD Vibrance Antifade Mounting Medium with DAPI (Vector Labs, Burlingame, CA, USA), as provided in the kit. Fluorescent images were evaluated within 48 h of mounting using a BZ-X710 fluorescence microscope (Keyence, Itasca, IL, USA).