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Ethylenediaminetetraacetic acid disodium salt dihydrate

Manufactured by Sinopharm
Sourced in China

Ethylenediaminetetraacetic acid disodium salt dihydrate is a chemical compound used as a chelating agent in various laboratory applications. It is commonly used to bind and sequester metal ions, such as calcium and magnesium, in order to control their reactivity and concentration in various experimental and analytical procedures.

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2 protocols using ethylenediaminetetraacetic acid disodium salt dihydrate

1

Synthesis and Characterization of Key Biochemical Reagents

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RDV (≥98%) was synthesized according to the literature [12 (link)]. The nucleotide monophosphate RMP (purity of 93%) and nucleotide triphosphate RTP (purity of 98%) were synthesized as previously reported [13 (link)]. Nucleoside RN (purity of 98%) was purchased from Target Molecule Corp (Boston, MA, USA). Dimethyl sulfoxide (DMSO), 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB, ≥ 98%), Trizma® base (Tris, ≥99.9%) and magnesium chloride hexahydrate (MgCl2·6H2O, 99%–102%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). PhosSTOP EASYpack (PhosSTOP) was purchased from Roche (Basel, Switzerland). Alamethicin was purchased from J&K Chemical, Ltd. (Beijing, China). Potassium chloride (KCl, ≥99.5%), ethylenediaminetetraacetic acid disodium salt dihydrate (Na2-EDTA·2H2O, ≥99.0%) and sucrose were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All other reagents were of the highest quality commercially available.
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2

Preparation and Characterization of Xanthine Oxidase Inhibitor

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NBT (98%) and allopurinol (98%) were purchased from Aladdin Industrial Corp. (Shanghai, China). XOD and xanthine (≥99%) were purchased from Sigma-Aldrich Chemical Corp. (St. Louis, MO, USA). Ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA-2Na·2H2O, ≥ 99.0%), dipotassium hydrogen phosphate trihydrate (K2HPO4·3H2O, ≥ 99.0%), potassium dihydrogen phosphate (KH2PO4, ≥99.5%), and dextran (Mw 20,000) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Glycerol (99%) was purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Bovine serum albumin (BSA) was purchased from Absin Bioscience Inc. (Shanghai, China). Trehalose (≥99%) was purchased from Sinozyme Biotechnology Co., Ltd. (Nanning, China). Ultrahigh-purity water was produced using a Milli-Q water purification system from Millipore (Milford, MA, USA). The 3D printing materials, namely, polycaprolactone, wax wire, and polylactic acid (PLA) filament, were purchased from Dongguan Top Cool Electronics Technology Co., Ltd. (Dongguan, China), Fuzhou Zhilei Electronic Technology Co., Ltd. (Fuzhou, China), and Hangzhou Shining 3D Technology Co., Ltd. (Hangzhou, China), respectively. Salvia miltiorrhiza (batch No. 170901) was purchased from Jiuzhou Drugstore (China Jo-Jo Drugstores Inc., Hangzhou, China).
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