Rat tnf α elisa kit

Of the two ovaries removed, one was fixed in 10% formaldehyde solution for histological examination, while the second was dry stored at −80°C after wrapping in aluminum foil for biochemical assays. Tumor necrosis factor-alpha (TNF-α), total antioxidant status (TAS), total oxidant status (TOS), and malondialdehyde (MDA) levels were determined in the supernatant following their respective methodology. Blood samples were centrifuged at 3,000 rpm for 10 min to separate the serum, which were then placed into Eppendorf tubes and stored at −80°C. Anti-mullerian hormone (AMH) assay was performed using rat AMH ELISA kits (Elabscience, catalogue no: E-EL-R0640). For TNF-α estimations, rat TNF-α ELISA kit (Boster Immuno leader, Boster Biological Technology Co., Ltd. Pleasanton, CA, USA, catalogue no; EK0526) was utilized. The ovarian tissues were prepared for homogenization by washing each tissue with cold saline. The resultant supernatants were removed and stored in sterile tubes at −80°C for tissue TAS and TOS analyses. An autoanalyzer (Siemens Advia 2400 Chemistry System, Siemens, Tokyo, Japan) and Reel Assay Total Antioxidant Status Test Kits were used for TAS and TOS determinations in accordance with standard kit procedures at the Central Laboratory of Firat University Hospital. All blood and tissue samples were stored and analyzed in the Firat University Biochemical Laboratory.

Expression of pro-inflammatory factors TNF-α and IL-6 was determined by Rat TNF-α ELISA kit (#EK0526) and Rat IL-6 ELISA kit (#EK0412) respectively, according to the manufactory’s protocol (Boster Biological Technology Co., Ltd.)