Nc sc 37007

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The NC (sc-37007) is a laboratory equipment product manufactured by Santa Cruz Biotechnology. It serves as a core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation.

Automatically generated - may contain errors

Lab products found in correlation

Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Btbd7 sirna sc 92326, by Santa Cruz Biotechnology (1 mentions) Peqgold rna extraction kits, by Avantor (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Human irf 1 sirna sc 35706, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Sc-37007 (308 citations) NC-siRNA (sc-37007) (12 citations)

3 protocols using nc sc 37007

A549 Cell Culture and NSCLC CSC Maintenance

Check if the same lab product or an alternative is used in the 5 most similar protocols

A549 cell lines were maintained in RPMI-1640 (Thermo Scientific, Waltham, MA, USA) with 10% (FBS; Thermo Scientific), which were provided by Cell Bank, Chinese Academy of Sciences (Shanghai, P.R. China). The NSCLC CSC line was maintained in a DMEM/F12 medium supplemented with ITS, 20 ng/ml EFG, and 20 ng/ml FGF. All cells were maintained in a humidified atmosphere of 5% CO₂ at 37°C. Btbd7-siRNA (sc-92326) and NC (sc-37007) were obtained from Santa Cruz Biotechnology (Dallas, TX, USA)

Fang L.Z., Zhang J.Q., Liu L., Fu W.P., Shu J.K., Feng J.G, & Liang X. (2017). Silencing of Btbd7 Inhibited Epithelial–Mesenchymal Transition and Chemoresistance in CD133+ Lung Carcinoma A549 Cells. Oncology Research, 25(5), 819-829.

+ Open protocol

+ Expand

Silencing of Sry and c-Myb in Human Osteoblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols

siRNAs for silencing of Sry (sc-38443), c-Myb (sc-29855) and a negative control, nc (sc-37007), were from Santa Cruz Biotechnology. Here, 30-pmol siRNA (final concentration, 300 nM) was nucleofected into 1 × 10⁶ human primary osteoblasts, as described above. After 24 h and 48 h of nucleofection, the growth medium was changed. Then, at 72 h after nucleofection, the RNA was isolated using PeqGold RNA extraction kits (VWR), reverse transcribed, and subjected to quantitative PCR for the genes of interest. Three biological replicates were analyzed.

Kodrič K., Zupan J., Kranjc T., Komadina R., Mlakar V., Marc J, & Lovšin N. (2019). Sex-determining region Y (SRY) attributes to gender differences in RANKL expression and incidence of osteoporosis. Experimental & Molecular Medicine, 51(8), 97.

+ Open protocol

+ Expand

Silencing human IRF-1 gene expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human IRF-1 siRNA (sc-35706) and negative control (NC, sc-37007) were purchased from Santa Cruz Biotech (CA, USA). The cells were seeded in a 6-well plate and the following day transfected siRNA (80 pmols) using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). Serum-free medium was replaced with growth medium after 8 h. 48 h after transfection, the protein level assay was performed.

Yan Y., Zheng L., Du Q., Yan B, & Geller D.A. (2020). Interferon regulatory factor 1 (IRF-1) and IRF-2 regulate PD-L1 expression in hepatocellular carcinoma (HCC) cells. Cancer immunology, immunotherapy : CII, 69(9), 1891-1903.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!