Trp53+/+, Trp53+/−, and Trp53−/− MEFs were treated with 10Gy irradiation to activate the p53 pathway. Equal quantities of cells from each group were pelleted, lysed with RIPA buffer (Sigma-Aldrich; Cat#: R0278-50ML), and utilized for immunoblotting experiments. Protein samples were collected from lysed cell pellets. Protein concentrations were normalized via both cell number and BCA assay (Thermo Fisher; Cat#: 23227). Samples were run through 4%–15% gradient polyacrylamide gels (BioRad; Cat#: 4561083EDU) and transferred onto nitrocellulose membranes (Thermo Fisher; Cat#: 88018). We used the Rabbit anti TRP53 primary antibody (Cell Signaling; Cat#: 9282) for detection of TRP53, along with goat anti-rabbit HRP-linked secondary antibody (Cell Signaling; Cat#: 7074S). Rabbit primary antibody was used for detection of ACTB (β-actin) (ABCAm: Cat#: ab8227), along with goat anti-rabbit HRP-linked secondary antibody (Cell Signaling; Cat#: 7074S) (Figure S1C ).
Rabbit anti trp53 primary antibody
Manufactured by Cell Signaling Technology
The Rabbit anti TRP53 primary antibody is a laboratory tool for detecting the presence and abundance of the TRP53 protein in biological samples. It is a polyclonal antibody raised in rabbits against the TRP53 protein, which is a key regulator of cellular processes such as cell cycle, apoptosis, and DNA repair. The antibody can be used in various immunodetection techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to identify and quantify the TRP53 protein in different cell and tissue types.
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Lab products found in correlation
Nitrocellulose membrane, by Thermo Fisher Scientific (2 mentions)
Goat anti rabbit hrp linked secondary antibody, by Cell Signaling Technology (2 mentions)
Gradient polyacrylamide gel, by Bio-Rad (1 mentions)
Bicinchoninic acid (bca), by Thermo Fisher Scientific (1 mentions)
Ripa buffer, by Merck Group (1 mentions)
2 protocols using rabbit anti trp53 primary antibody
1
p53 Pathway Activation Immunoblotting
2
Analysis of p53 Activation in MEFs
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Trp53 +/+ , Trp53 +/-, and Trp53 -/-MEFs were treated with 10Gy irradiation to activate the p53 pathway. Equal quantities of cells from each group were pelleted, lysed with RIPA buffer, and utilized for immunoblotting experiments. Protein samples were collected from cell pellets lysed with RIPA buffer. Protein concentrations were normalized via both cell number and BCA assay. Samples were run through 4-15% gradient polyacrylamide gels (BioRad; Cat#: 4561083EDU) and transferred onto nitrocellulose membranes (Thermo Fisher; Cat#: 88018). We used the Rabbit anti TRP53 primary antibody (Cell Signaling; Cat#: 9282) for detection of TRP53, along with goat anti-rabbit HRP-linked secondary antibody (Cell Signaling; Cat#: 7074S). Rabbit primary antibody was used for detection of ACTB (B-actin) (Abcam: Cat#: ab8227), along with goat anti-rabbit HRP-linked secondary antibody (Cell Signaling; Cat#: 7074S) (Figure S1C).
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