The following primary and secondary antibodies were used for immunofluorescence: anti-E-cadherin rat antibody (#13-1900, 1:50 dilution, Thermo Fischer Scientific), anti-SOX9 rabbit antibody (#AB5535-25UG, 1:200 dilution, Merck Millipore), Alexa Fluor 546-conjugated goat anti-rat IgG (H+L) antibody (#A11081, 1:1000 dilution), Alexa Fluor 647-conjugated goat anti-rat IgG (H+L) antibody (#A21247, 1:1000 dilution), Alexa Fluor 647-conjugated goat anti-rabbit IgG (H+L) antibody (#A32733, 1:1000 dilution)(all Thermo Fisher Scientific).
Alexa fluor 647 conjugated goat anti rat igg h l antibody
Manufactured by Thermo Fisher Scientific
Alexa Fluor 647-conjugated goat anti-rat IgG (H+L) antibody is a secondary antibody that binds to the heavy and light chains of rat immunoglobulin G (IgG) molecules. The antibody is conjugated with Alexa Fluor 647, a fluorescent dye that can be detected using instruments capable of detecting near-infrared fluorescence.
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Lab products found in correlation
Rabbit anti sox9 antibody, by Merck Group (2 mentions)
Alexa fluor 546 conjugated goat anti rat igg h l antibody, by Merck Group (2 mentions)
Alexa fluor 647 conjugated goat anti rabbit igg h l antibody, by Thermo Fisher Scientific (2 mentions)
Anti e cadherin rat antibody, by Thermo Fisher Scientific (1 mentions)
Anti ecadherin rat antibody, by Cell Signaling Technology (1 mentions)
2 protocols using alexa fluor 647 conjugated goat anti rat igg h l antibody
1
Immunofluorescence Labeling of E-cadherin and SOX9
2
Immunofluorescence Imaging of E-cadherin and SOX9
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The following primary and secondary antibodies were used for immunofluorescence: anti-Ecadherin rat antibody (#3195, 1:100 dilution, Cell Signaling Technology), anti-SOX9 rabbit antibody (#AB5535-25UG, 1:200 dilution, Merck Millipore), Alexa Fluor 546-conjugated goat anti-rat IgG (H+L) antibody (#A11081, 1:1000 dilution), Alexa Fluor 647-conjugated goat anti-rat IgG (H+L) antibody (#A21247, 1:1000 dilution), Alexa Fluor 647-conjugated goat anti-rabbit IgG (H+L) antibody (#A32733, 1:1000 dilution)(all Thermo Fisher Scientific).
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