Healthy 1-month-old Landrace piglets were purchased from Jilin University Breeding pig farm, porcine PBMECs isolated, and the BBB model constructed as described previously [21 (link)]. The stability of the BBB model was assessed by measurement of transendothelial cell electric resistance (TEER), using the Millicell-ERS voltage resistance meter (Merck Millipore, Burlington, VT, USA) as per the manufacturer’s instructions. Each cell culture well was measured three times and averaged, and reported as Ω·cm2 after correcting for the surface area of the membrane. TEER values of PBMEC cultures were monitored from day 2 to day 8 of the experiments.
The integrity of the BBB model was evaluated by TEER as described previously [21 (link)]. HSPD1 (20 μg/mL) was added to the upper chamber of the BBB model, and PBS alone was used as the control group. The TEER values were determined at 0 h, 1 h, 2 h, 3 h, 6 h, 9 h, 12 h, 18 h, and 24 h, using the Millicell-ERS voltage resistance meter (Merck Millipore, Burlington, VT, USA) as per the manufacturer’s instructions. The TEER of each cell culture well was measured three times and averaged, and reported as Ω·cm2 after correcting for the surface area of the membrane.
The integrity of the BBB model was evaluated by TEER as described previously [21 (link)]. HSPD1 (20 μg/mL) was added to the upper chamber of the BBB model, and PBS alone was used as the control group. The TEER values were determined at 0 h, 1 h, 2 h, 3 h, 6 h, 9 h, 12 h, 18 h, and 24 h, using the Millicell-ERS voltage resistance meter (Merck Millipore, Burlington, VT, USA) as per the manufacturer’s instructions. The TEER of each cell culture well was measured three times and averaged, and reported as Ω·cm2 after correcting for the surface area of the membrane.