Human mesenchymal stem cells (MSC) and osteoblasts were obtained from Lonza Group Ltd (hereafter referred to as “Lonza”). Mesenchyme cells were grown and maintained in Mesenchymal Cell Growth Supplement (MCGS, PT-3001) with L-glutamine and containing 30 mg/ml gentamicin, 15 ug/ml amphotericin and 10% fetal bovine serum (FBS). Cells were cultured at 37 °C in the presence of 5% CO2 until ∼75% confluent. Mesenchyme cells were induced to differentiate to osteoblasts by culturing the cells in the presence of osteogenic medium from Lonza according to the manufacturer's protocol. Human primary osteoblasts were also obtained from Lonza. The primary osteoblasts were cultured in Clonetics Osteoblast Growth Medium especially formulated for the growth and expansion of human osteoblasts, and contained 10% FBS, ascorbic acid and gentamicin-amphotericin.
Osteoblasts
Manufactured by Lonza
Osteoblasts are primary bone cells responsible for the formation and mineralization of bone. They secrete the organic matrix of bone, known as osteoid, which subsequently becomes mineralized through the deposition of calcium and phosphate crystals. Osteoblasts play a crucial role in bone remodeling and maintenance.
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Lab products found in correlation
2 protocols using osteoblasts
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Differentiation of Mesenchymal Stem Cells to Osteoblasts
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Differentiation of Mesenchymal Stem Cells to Osteoblasts
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Human mesenchymal stem cells (MSC) and osteoblasts were obtained from Lonza Group Ltd (hereafter referred to as “Lonza”). Mesenchyme cells were grown and maintained in Mesenchymal Cell Growth Supplement (MCGS, PT-3001) with L-glutamine and containing 30 mg/ml gentamicin, 15 ug/ml amphotericin and 10% fetal bovine serum (FBS). Cells were cultured at 37 °C in the presence of 5% CO2 until ∼75% confluent. Mesenchyme cells were induced to differentiate to osteoblasts by culturing the cells in the presence of osteogenic medium from Lonza according to the manufacturer's protocol. Human primary osteoblasts were also obtained from Lonza. The primary osteoblasts were cultured in Clonetics Osteoblast Growth Medium especially formulated for the growth and expansion of human osteoblasts, and contained 10% FBS, ascorbic acid and gentamicin-amphotericin.
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