Liver tissues were embedded in paraffin and 5‐µm‐thick sections were prepared. The sections were placed on slides and heated at 60°C for 2 hours. Following dewaxing, rehydration, the slides were incubated in boiled antigen retrieval buffer for 10 minutes. Then slides were blocked with goat serum for 15 minutes at room temperature. For double labelling, slides were incubated with desmin antibody (1:50; Proteintech, Rosemont, IL) plus proliferating cell nuclear antigen (PCNA) antibody (1:50; Santacruz Biotechnology, Santa Cruz, CA) at 4°C overnight, followed by incubation with fluorescein isothiocyanate (FITC)‐conjugated anti‐mouse IgG (1:200; Beyotime, Haimen, China) and Cy3‐conjugated anti‐rabbit IgG (1:200; Beyotime) for 90 minutes at room temperature. Finally, the slides were counterstained with 4′,6‐diamidino‐2‐phenylindole (DAPI) and observed under a fluorescence microscope (BX53; Olympus).
Proliferating cell nuclear antigen antibody
Manufactured by Santa Cruz Biotechnology
Sourced in Israel
The Proliferating Cell Nuclear Antigen (PCNA) antibody is a laboratory reagent used for the detection and quantification of PCNA in various biological samples. PCNA is a protein that plays a crucial role in DNA replication and repair processes. The PCNA antibody can be used in techniques such as Western blotting, immunohistochemistry, and flow cytometry to study PCNA expression and localization in cells.
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Lab products found in correlation
2 protocols using proliferating cell nuclear antigen antibody
1
Liver Tissue Immunofluorescence Analysis
2
Integrin-Mediated Apoptosis Signaling Assay
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T 3 , T 4 and tetrac (Sigma-Aldrich) were dissolved in DMSO to 100 mM and further dissolved in 1 mM KOH-propylene glycol, which was also used as a vehicle control (final concentration of 0.04 N KOH with 0.4% polyethylene glycol (vol/vol)). RGD and Arg-Gly-Glu (RGE) peptides (Sigma-Aldrich) were dissolved at 100 mM in PBS. Bortezomib (dissolved in saline to 2.6 µM) was obtained from the oncology pharmacy at Meir Medical Center. The volume of solvent added to each well has been kept constant for all conditions. Pan-caspase inhibitor, Z-VAD-FMK was purchased from Enzo Life Sciences (Farmingdale, NY, USA). Monoclonal antibody against αvβ3 integrin (LM609, PE/FITC-conjugated) was from Chemicon International. APC-CD138 antibodies were from Miltenyi Biotec (Bergisch Gladbach, Germany), FITC-CD45 and PE-CD38 antibodies were from BioLegend, San Diego, CA, USA. Proliferating cell nuclear antigen (PCNA) antibody was from Santa Cruz Biotechnology. Antibodies against total and cleaved caspase-9, caspase-3, PARP-1 and tubulin were from Cell Signaling. Anti-AIF was from EMD Millipore. Anti-pATM (phospho-serine 1981) was from Epitomics (Burlingame, CA, USA) and anti-pγH2AX (phospho-serine 139) from Chemicon International. All antibodies were appropriately validated.
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