Protein expression levels were determined using Western blot analysis. The protein samples were resolved by 10% SDS-PAGE and transferred to polyvinylidene fluoride membranes (BIO-RAD, United States), which were then incubated overnight at 4°C in the following primary antibodies: anti-PS1 (Thermo Fisher Scientific, cat. no. PA5-98093, United States), and anti-GAPDH (CST, cat. no. 5174, United States). Three independent experiments that included both technical and biological replicates were performed. To explore Aβ levels, Aβ40, and Aβ42 ELISA kits (Invitrogen, United States) were used according to the manufacturer’s instructions. In brief, the cell media were mixed with Aβ 40-, or Aβ42-specific antibody and shake-incubated in a 96-well plate overnight at 4°C. After incubation with secondary antibody for 30 min, the reaction with substrate was carried out at room temperature. The color intensity was measured at 450 nm using a multimode plate reader (EnVision®, Perkin Elmer, United States). Five independent experiments that included both technical and biological replicates were performed.
Anti ps1
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-PS1 is a laboratory product designed for the detection and quantification of Presenilin-1 (PS1), a key component of the gamma-secretase complex involved in the production of amyloid-beta peptides. This product enables researchers to study the role of PS1 in various biological processes and disease states.
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Lab products found in correlation
2 protocols using anti ps1
1
Protein Expression and Aβ Quantification
2
Immunohistochemical Localization of Proteins
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Isolated vessels were incubated in tri-sodium citrate buffer 10 mM, pH 6 and heated in a microwave (900W) for 20s for antigen retrieval. Primary antibodies were diluted 1:200 in phosphate buffer (PB: 0.1-M NaH 2 PO 4 and 0.1-M Na 2 HPO 4 ), donkey serum 2%, triton 0.2%, BSA 0.1%, and incubated overnight at 4 C. Anti-PS1 (antibody registry: AB_2170605) and anti-PS2 (antibody registry: AB_325093) were from Thermo Scientific. Anti-MLC2 (myosine light chain 2 specific of smooth muscle, antibody registry: AB_2148039) was from Clinisciences. Anti-smoothelin (antibody registry: AB_2193677) was from Santa Cruz. Anti-NeuN (antibody registry: AB_2149209) was from Millipore. Anti-GFAP (antibody registry: AB_2532994) was from Thermo Fischer. Secondary antibodies (from Clinisciences) were incubated 2 hours at room temperature and washed 3 times. Slices and vessels are mounted in Vectashield hard-set or Fluoromount and observed with a confocal microscope (LEICA SP5) or macroscope (LEICA LSI).
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