Serum concentration of IL-17, IL-21, IL-23, IL-35, and Foxp3 proteins was measured according to the manufacturers’ protocols with the use of Human IL-17 Quantikine ELISA Kit, Human IL-23 Quantikine ELISA Kit (R&D Systems, Inc. Minneapolis, MN, USA), Human IL21 ELISA Kit (CLIA) from LifeSpan BioSciences (Biocompare South San Francisco, CA, USA), Human IL-35 ELISA Kit and Human FOXP3 ELISA Kit (MyBioSource, Inc., San Diego, CA, USA). An endogenous control of protein concentration in the samples collected from the patients was performed with β-actin. Human Actin Beta (ACTb) ELISA Kit (BMASSAY) was used in this case in line with the recommendations specified by manufacturer. Multiskan Ascent Microplate Photometer (Thermo Labsystems) allowed the absorbance of the samples to be measured and analyzed at λ = 450 nm. Analytical curves for the proteins were prepared with the aim of determining protein concentration.
Human foxp3 elisa kit
Manufactured by MyBioSource
Sourced in United States
The Human FOXP3 ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human FOXP3 in biological samples such as cell culture supernatants and cell lysates.
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2 protocols using human foxp3 elisa kit
1
Quantifying Immune Biomarkers in Patients
2
Quantifying Immune Biomarkers in Serum
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A 10 ml of fasting venous blood was taken from each patient for laboratory assessment.An auto-analyzer BS200 (Mindray, Shenzhen, China) was used.In this context,biochemical examination including fasting blood sugar (FBS), total cholesterol (Chol), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), triglycerides (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were done by using Pars Azmoon Company (Pars Azmoon Co., Tehran, Iran) commercial diagnostic kits.
Human FOXP3 ELISA Kit (MBS2503897, Mybiosource Co., USA) was used for in vitro quantitative determination of human FOXP3 concentrations in serum by using a quantitative sandwich method according to the manufacturer’s protocol. According to the protocol, ELISA kit for NFkB (MyBioSource, San Diego, California, United States) was used.
Human FOXP3 ELISA Kit (MBS2503897, Mybiosource Co., USA) was used for in vitro quantitative determination of human FOXP3 concentrations in serum by using a quantitative sandwich method according to the manufacturer’s protocol. According to the protocol, ELISA kit for NFkB (MyBioSource, San Diego, California, United States) was used.
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