All chemicals are from Sigma-Aldrich (highest grade) if not mentioned otherwise. The following antibodies and reagents were purchased from Biolegend: APC/Cy7 anti-human CD3 antibody, BV421 anti-human CD3 antibody, BV421 anti-human CD8 antibody, APC anti-human CD253 (TRAIL) antibody, APC anti-mouse CD253 (TRAIL) Antibody, BV421 anti-mouse CD8a Antibody, PE anti-mouse CD3 Antibody, PerCP anti-human CD25 antibody, APC anti-human CD62L antibody, APC anti-human CD262 (TRAIL-R2), and 7-AAD viability staining solution. The following antibodies were also used: FITC anti-human CD69 (eBiosciences), FITC anti-human CD44 (DAKO), Purified NA/LE mouse anti-human CD253 (BD Biosciences), BV421 mouse anti-human CD263 (TRAIL-R3) (BD Biosciences), Alexa647 mouse anti-human GLUT1 (BD Biosciences), human TRAIL R1/TNFRSF 10A PerCP-conjugated antibody (R&D Systems), and human TRAIL R4/TNFRSF 10D PE-conjugated antibody (R&D Systems). In addition, the following reagents were used: NucView Caspase-3 enzyme substrates (Biotium), Idelalisib (Selleckchem), MK-2206 (Selleckchem), Rapamycin (Selleckchem), Caffeic acid phenethyl ester (CAPE) (R&D Systems), Mitoquinone (MitoQ) (Biotrend), N-acetyl-L-cysteine (NAC) (Merck), vitamin 1,25D3 (Merck), Calcipotriol (TOCRIS), DMSO (Merck), Cellular ROS Assay Kit (Abcam), metformin hydrochloride (Merck), H2O2 (Merck), and Streptozotocin (Merck).
Bv421 anti mouse cd8a antibody
Manufactured by BioLegend
The BV421 anti-mouse CD8a Antibody is a fluorescently-labeled monoclonal antibody that binds to the CD8a surface antigen expressed on a subset of T lymphocytes in mice. This antibody can be used for the identification and enumeration of CD8+ T cells in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Idelalisib, by Selleck Chemicals (1 mentions)
Metformin hydrochloride, by Merck Group (1 mentions)
Streptozotocin (stz), by Merck Group (1 mentions)
Rapamycin, by Selleck Chemicals (1 mentions)
Pe anti mouse cd3 antibody, by BioLegend (1 mentions)
Apc anti human cd62l antibody, by BioLegend (1 mentions)
Caffeic acid phenethyl ester cape, by R&D Systems (1 mentions)
N acetyl l cysteine nac, by Merck Group (1 mentions)
Apc cy7 anti human cd3 antibody, by BioLegend (1 mentions)
Cellular ros assay kit, by Abcam (1 mentions)
Mk 2206, by Selleck Chemicals (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Live dead kit, by Thermo Fisher Scientific (1 mentions)
Lsrii ow cytometer, by BD (1 mentions)
Anti cd45 antibody, by BioLegend (1 mentions)
Pe anti mouse cd4 antibody, by BioLegend (1 mentions)
2 protocols using bv421 anti mouse cd8a antibody
1
Comprehensive Immune Cell Profiling and Targeting in T Cell Activation
2
Murine Immune Cell Profiling by Flow Cytometry
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Single-cell suspensions of spleen cells and peripheral blood mononuclear cells (PBMCs) from mice were prepared. Sample cells were concentrated on centrifugation at 250 g for 5 min at 4°C and incubated in 2 ml of erythrocyte lysates (1:10, BD555899, Biolegend). The cells were washed in phosphate buffered saline (PBS) prior to staining for ow cytometry. The cells were then labeled with dead cells (Live/Dead kit, Invitrogen) to gate out the dead cells and stained with antibodies speci c to anti-CD45 antibody (103108, Biolegend), PerCP/Cy5.5 anti-mouse CD3 antibody (100218, Biolegend), PE anti-mouse CD4 antibody (100408, Biolegend), BV421 anti-mouse CD8a antibody (100738, Biolegend). The samples were incubated for 30 min at room temperature. Cells were xed in 4% formaldehyde and washed twice with PBS and acquired via ow cytometry within 24 hours. All ow cytometry experiments were performed on an LSRII ow cytometer (BD Biosciences).
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