β3 tubulin rabbit polyclonal and mouse monoclonal antibody

Manufactured by Fortrea

β3-tubulin rabbit polyclonal and mouse monoclonal antibody is a laboratory reagent used for the detection and quantification of β3-tubulin, a member of the tubulin protein family, in biological samples. The rabbit polyclonal and mouse monoclonal antibodies are specific to the β3-tubulin isoform and can be used in various analytical techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry.

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Lab products found in correlation

Gapdh mouse monoclonal antibody, by Abcam (2 mentions) Appropriate fluorescent secondary antibodies, by Jackson ImmunoResearch (1 mentions)

2 protocols using β3 tubulin rabbit polyclonal and mouse monoclonal antibody

Antibody Characterization for Neuronal Cytoskeleton

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Rabbit polyclonal antibody against MAP2 was provided by Itzhak Fischer (Drexel University College of Medicine, Philadelphia, PA; Nunez and Fischer, 1997 (link)). Other antibodies were purchased as noted: detyrosinated tubulin rabbit polyclonal antibody (Millipore), acetylated tubulin mouse monoclonal antibody (Sigma-Aldrich), tyrosinated tubulin YL1/2 rat monoclonal antibody (Accurate Scientific, Westbury, NY), β3-tubulin rabbit polyclonal and mouse monoclonal antibody (Covance, Princeton, NJ), β-tubulin fluorescein isothiocyanate–conjugated monoclonal mouse antibody (Sigma-Aldrich), kinesin-5 phosphorylated at Thr-926 rabbit polyclonal antibody (Phospho Solutions, Aurora, CO), total kinesin-5 rabbit polyclonal antibody (Abcam, Cambridge, UK), and mouse monoclonal GAPDH antibody (Abcam). Appropriate fluorescent secondary antibodies were purchased from Jackson Immunoresearch (West Grove, PA).

Kahn O.I., Sharma V., González-Billault C, & Baas P.W. (2015). Effects of kinesin-5 inhibition on dendritic architecture and microtubule organization. Molecular Biology of the Cell, 26(1), 66-77.

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Immunofluorescence Staining of Neurons

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Rabbit polyclonal antibody against MAP2 was provided by Dr. Itzhak Fischer, Drexel University College of Medicine, Philadelphia, PA (Nunez and Fischer, 1997 (link)). Other antibodies were purchased as follows: β3-tubulin rabbit polyclonal and mouse monoclonal antibody (Covance), β-tubulin cy3 conjugated monoclonal mouse antibody (Sigma), TPX2 rabbit polyclonal antibody (Santa Cruz Biotechnology), mouse monoclonal actin antibody (Abcam), mouse monoclonal GAPDH antibody (Abcam). Appropriate fluorescent goat secondary antibodies were purchased from Jackson Immunoresearch.

Kahn O.I., Ha N., Baird M.A., Davidson M.W, & Baas P.W. (2015). TPX2 regulates neuronal morphology through kinesin-5 interaction. Cytoskeleton (Hoboken, N.J.), 72(7), 340-348.

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