AML cell lines HL-60 (ACC-3)[30 (link)], KG-1 (ACC-14)[31 (link)], MonoMac-1 (ACC-252)[32 (link)] and Kasumi-1 (ACC-220)[33 (link)] were obtained from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen) and the human stroma cell line HS-5 was purchased from ATCC (American Type Culture Collection). Experiments were performed within 6 months after receipt or resuscitation. AML cell lines were cultured in complete RPMI medium (PAA laboratories) supplemented with fetal bovine serum (Lonza), sodium pyruvate (Lonza) and/or non-essential amino acids (Lonza) according to manufacturers' recommendations. HS-5 cell line was cultured in complete DMEM medium (PAA laboratories) supplemented with 10% fetal bovine serum (Lonza). Primary AML blasts were cultured in IMDM (PAA laboratories) supplemented with 3% heat-inactivated fetal bovine serum (Lonza), 1x BIT (StemCell Technologies), 5 ng/ml IL3 (Peprotech), sodium pyruvate (Lonza) and β-mercaptoethanol (Sigma).
Complete dmem medium
Manufactured by GE Healthcare
Complete DMEM medium is a culture medium used for the maintenance and growth of a variety of cell lines in cell culture applications. It provides the necessary nutrients, growth factors, and other components to support the proliferation and viability of cells.
Automatically generated - may contain errors
Lab products found in correlation
Non essential amino acids (neaa), by Lonza (2 mentions)
Complete rpmi medium, by GE Healthcare (2 mentions)
Sodium pyruvate, by Lonza (2 mentions)
Fetal bovine serum (fbs), by Lonza (2 mentions)
Interleukin 3 (il 3), by Thermo Fisher Scientific (1 mentions)
Hl 60, by Leibniz Institute DSMZ (1 mentions)
Kasumi 1, by Leibniz Institute DSMZ (1 mentions)
β mercaptoethanol, by Merck Group (1 mentions)
Monomac 1, by Leibniz Institute DSMZ (1 mentions)
Monomac 1 acc 252, by Leibniz Institute DSMZ (1 mentions)
Kasumi 1 acc 220, by Leibniz Institute DSMZ (1 mentions)
2 protocols using complete dmem medium
1
Culturing AML Cell Lines and Primary Blasts
2
Culture and Co-culture of AML Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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AML cell lines HL60 (ACC-3) [13 (link)], KG-1 (ACC-14) [14 (link)], MonoMac-1 (ACC-252) [15 (link)] and Kasumi-1 (ACC-220) [16 (link)] were obtained from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen) and the human stroma cell line HS-5 was purchased from ATCC (American Type Culture Collection). Experiments were performed within 6 months after receipt or recovery after thawing. AML cell lines were cultured in complete RPMI medium (PAA laboratories) supplemented with 10 % fetal bovine serum (Lonza), sodium pyruvate (Lonza) and non-essential amino acids (Lonza) according to manufacturers’ recommendations. HS-5 cell line was cultured in complete DMEM medium (PAA laboratories) supplemented with 10 % fetal bovine serum (Lonza) prior to co-culture experiments. Co-culture experiments were performed in complete RPMI medium as described for AML cells.
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