Xn hematology analyzer

This study was conducted in compliance with the ethical principles of the Declaration of Helsinki. This study was reviewed and approved by the Boston Children’s Hospital Committee on Clinical Investigation (protocol # AX-09-0503-4) and all subjects provided written informed consent. Healthy volunteers were allowed to enroll in the study if they were aged ≥18 years, free of aspirin or other antiplatelet medication for ≥10 days, and free of all other non-steroidal anti-inflammatory drugs for ≥ 3 days. Following a 2 mL discard, 120 mL of blood was collected from each of 5 volunteers into 1/10^th volume of acid-citrate-dextrose solution A (ACD-A). PRP was prepared as previously described [15 (link)] according to the manufacturer’s recommendation using the Harvest^® SmartPreP2 System (Harvest Technologies, Plymouth, MA, USA) with two 60 mL cartridges. The resultant PRP was pooled prior to further treatment. Complete blood cell counts were performed on the ACD-anticoagulated whole blood and the concentrated PRP in a Sysmex XN Hematology Analyzer.

A HeaLink R211B micro-electrocardiogram (ECG) recorder (Healink Ltd., Bengbu, China) was used 1 day prior to surgery to collect ECG data (5 min) from CC patients while in a supine position. During the ECG, the test environment was kept quiet and the patients were asked to relax and breathe smoothly. Measurements were obtained with disposable Ag/AgCl gel electrodes (JunKang Ltd., Shanghai, China). ECG signals were amplified on the device (bandwidth 0.67–40 Hz) and digitized with a 400 Hz sampling rate.
On post-operative day 2, median cubital vein blood was collected early in the morning, and neutrophil, lymphocyte, monocyte, and platelet counts were analyzed using a Sysmex XN hematology analyzer (Sysmex, Kobe, Japan). Based on the findings, LMR, NLR, and PLR values were calculated.

Analyses were performed on residual EDTA K3-anticoagulated blood samples initially collected for blood cell count as part of standard care. For both patients and controls, routine blood cell counts were performed on Sysmex XN^™ hematology analyzer (Sysmex France, Villepinte, France). In addition, samples were analyzed with a mock-up derived from the ABX Micros 60 (HORIBA Medical), for the recording of the electrical pulses induced by RBC in an aperture-electrode system (also called Coulter-based system). The pulses acquisition is performed by a LabVIEW^™ (National Instruments) code, as presented before [3 (link), 4 (link)]. The mock-up differs from the commercial version ABX Micros 60, with an electronic bandwidth increased to 150 kHz. This modification guarantees a high-quality signal and a reliable electrical pulse processing. Commercial ABX Diluent was used (HORIBA Medical). Unlike some routine hematological analyzers, there is no need to spherize RBC prior acquisition and no hydrodynamical focusing is used with this device. All acquisitions were performed at room temperature. The original parameters derived from the pulse’s acquisitions are introduced in the following section.