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Disodium hydrogen phosphate na2hpo4

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Disodium hydrogen phosphate (Na2HPO4) is a chemical compound commonly used in various laboratory applications. It is a white, crystalline solid that is soluble in water and has a neutral pH. The primary function of disodium hydrogen phosphate is to serve as a buffer, maintaining a specific pH level in solutions. It is commonly used in biochemical, analytical, and cell culture applications.

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3 protocols using disodium hydrogen phosphate na2hpo4

1

Fatty Acid Extraction and Identification

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Acetonitrile, chloroform, ethyl acetate and methanol were purchased from J. T. Baker (München, Germany), and D-chloroform, formic acid 98%, Casein-Peptone Soymeal-Peptone (CASO), Potato Dextrose (PD), Luria–Bertani broth (LB), sodium chloride (NaCl), potassium chloride (KCl), potassium dihydrogen phosphate (KH2PO4), D-methanol and trifluoroacetic acid (TFA) were purchased from Carl Roth GmbH (Karlsruhe, Germany). Bacto malt extract, Bacto peptone and agar were from BD (La Point de Claix, France), and D-glucose was from Merck (Darmstadt, Germany). Disodium hydrogen phosphate (Na2HPO4) was purchased from J. T. Baker (The Netherlands), crystal violet from Fluka (Steinheim, Germany) and tetracycline and Potato Dextrose agar (PDA) from Sigma Aldrich (Taufkirchen, Germany). The fatty acids hexadecanoic acid (palmitic acid), (9E)-9-hexadecenoic acid (palmitelaidic acid), (9Z)-9-hexadecenoic acid (palmitoleic acid), (9Z)-9-Octadecenoic acid (oleic acid), (9Z,12Z)-octadeca-9,12-dienoic acid (linoleic acid), (6Z,9Z,12Z)-octadeca-6,9,12-trienoic acid (γ-linolenic acid) and (5Z,8Z,11Z,14Z)-Eicosa-5,8,11,14-tetraenoic acid (arachidonoic acid) were purchased from Sigma-Aldrich, St. Louis, MO, USA, while (7Z,10Z)-hexadeca-7,10-dienoic acid came from Larodan Inc., Monroe, LA, USA.
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2

Extraction and Analysis of Microbial Metabolites

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Acetonitrile, chloroform, ethyl acetate and methanol were purchased from J.T.Baker (München, Germany) respectively, d-chloroform, formic acid 98%, Potato Dextrose (PD), Luria-Bertani broth (LB), sodium chloride (NaCl), potassium chloride (KCl), potassium dihydrogen phosphate (KH2PO4), d-methanol, and trifluoroacetic acid (TFA) from Carl Roth GmbH (Karlsruhe, Germany). Bacto malt extract, Bacto peptone and agar were from BD (La Point de Claix, France), d-glucose from Merck (Darmstadt, Germany). Disodium hydrogen phosphate (Na2HPO4) was purchased from J.T.Baker® (The Netherlands), crystal violet from Fluka (Steinheim, Germany), tetracycline and potato dextrose agar (PDA) from Sigma Aldrich (Taufkirchen, Germany) respectively.
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3

Analytical Reagents for Microbiological Research

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Acetonitrile, chloroform, ethyl acetate and methanol were purchased from J. T. Baker (München, Germany) respectively, D-chloroform, formic acid 98%, Casein-soja-peptone (CASO), Potato Dextrose (PD), Luria-Bertani broth (LB), sodium chloride (NaCl), potassium chloride (KCl), potassium dihydrogen phosphate (KH2PO4), D-methanol, and trifluoroacetic acid (TFA) from Carl Roth GmbH (Karlsruhe, Germany). Bacto malt extract, Bacto peptone and agar were from BD (La Point de Claix, France), D-glucose from Merck (Darmstadt, Germany). Disodium hydrogen phosphate (Na2HPO4) was purchased from J.T. Baker® (Deventer, The Netherlands), crystal violet from Fluka (Steinheim, Germany), tetracycline and Potato-Dextrose agar (PDA) from Sigma Aldrich (Taufkirchen, Germany) respectively.
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